Plant Vitamin B7 (VB7) ELISA Kit

The ELISA kit applies the double-antibody sandwich method to determine the level of Plant Vitamin B7 (VB7)in plant samples. Purified Plant Vitamin B7 (VB7)antibodies are coated onto a microplate to form a solid-phase antibody. To these coated monoclonal antibody wells, Plant Vitamin B7 (VB7)is added, followed by an HRP-conjugated Plant Vitamin B7 (VB7)antibody, forming an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added and turns blue under the catalysis of HRP, and then changes to a final yellow color due to the addition of acid. The color intensity is directly proportional to the amount of Plant Vitamin B7 (VB7)present in the sample. The absorbance (OD value) is measured at 450 nm using an ELISA reader, and the concentration of Plant Vitamin B7 (VB7)in the plant samples is calculated based on a standard curve.

• Supplier: Lifeasible
• Product #: PEK-048
• specs: 96T/48T