Recombinant Human DNASE1L3 protein, GST-tagged
The cDNA fragment encoding 21-305aa of Human Deoxyribonuclease gamma (DNASE1L3) was fused with an N-terminal GST-tag and then expressed in E.coli. The product obtained is the recombinant full-length of mature human DNASE1L3 protein. Its purity was determined by using SDS-PAGE and reached up to 90%. Under the reducing conditions, the gel presented a molecular mass band of about 62 kDa. The slightly higher result was attributed to glycosylation. It was also validated by the LC-MS/MS analysis. In-stock DNASE1L3 proteins are offered now. This recombinant DNASE1L3 protein may find uses in the specific antibody generation or the studies of cell biology.
DNASE1L3 is a secreted DNASE1-like nuclease, which can digest DNA in chromatin. The deletion of DNASE1L3 can cause anti-DNA responses and autoimmunity in humans and mice. Lee Serpas et al. proved that DNASE1L3 plays a role in circulating plasma DNA homeostasis by enhancing fragmentation and affecting terminal motif frequencies. These results support the unique role of DNASE1L3 as a regulator of physical form and cell-free DNA availability, which may be significant for the DNASE1L3's mechanism of preventing autoimmunity.
DNASE1L3 is a secreted DNASE1-like nuclease, which can digest DNA in chromatin. The deletion of DNASE1L3 can cause anti-DNA responses and autoimmunity in humans and mice. Lee Serpas et al. proved that DNASE1L3 plays a role in circulating plasma DNA homeostasis by enhancing fragmentation and affecting terminal motif frequencies. These results support the unique role of DNASE1L3 as a regulator of physical form and cell-free DNA availability, which may be significant for the DNASE1L3's mechanism of preventing autoimmunity.
| Supplier | Creative Biomart |
|---|---|
| Product # | DNASE1L3-12097H |
| Pricing | 25ug : USD $319 |