DNA dA-Tailing Kit

The DNA dA-Tailing Kit efficiently adds a non-template dAMP (dA) to the 3＊ end of a blunt-ended DNA fragment. This incorporated 3＊-dA prevents concatemer formation and prepares the DNA fragment for subsequent ligation of adaptors or cloning vectors that have complementary 3＊-dT overhangs. The Klenow Fragment (3∩↙ 5∩ Exo-) in this kit adds the 3＊-dA via its DNA polymerase activity. This enzyme lacks 5＊↙3＊ exonuclease activity and has mutations that effectively abolish the inherent 3＊↙5＊ exonuclease activity, thus preventing degradation of this 3＊-dA. This kit has been optimized to prepare compatible overhangs for the next step of DNA sample preparation for next generation sequencing. The Klenow Fragment (3∩↙ 5∩ Exo-) enzyme is also available as a stand-alone enzyme (Cat. No. E038).

• Supplier: Amerigo Scientific
• Product #: APPL_E009