Recombinant Human LIF Protein

Recombinant Human LIF Protein is produced by E. coli. It is a single non-glycosylated polypeptide chain containing 180 amino acids with a molecular mass of 19.7 kD.

• Supplier: Cohesion Biosciences
• Product #: CRP1900
• Source: E. coli
• Reactivity: Human
• Applications: E, WB, SDS-PAGE, MS
• Delivery Time: Ship in 1 week
• Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 0.02% Tween 20, pH 7.4
• Gene Symbol: LIF
• Alternative Names: HILDA; Leukemia inhibitory factor; LIF; Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor; MLPLI; Emfilermin
• Entrez Gene (Human): 3976.0
• SwissProt (Human): P15018
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Quality Control: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage/Stability: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Datasheet: http://www.cohesionbio.com/download/CRP1900.pdf