Sonic Hedgehog/SHH Protein, Human, Recombinant (aa 198-462, His Tag)

Supplier Ace Therapeutics
Product # NSC1115
Pricing Inquiry
Product Size 100 µg
Purity > 95 % as determined by SDS-PAGE
Product Type Proteins
Synonyms HHG1 Protein, Human; HLP3 Protein, Human; HPE3 Protein, Human; MCOPCB5 Protein, Human; SMMCI Protein, Human; Sonic hedgehog Protein, Human; TPT Protein, Human; TPTPS Protein, Human
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Activity Testing in progress
Protein Construction A DNA sequence encoding the amino acid sequence (Cys 198-Ser 462) of human SHH (Q15465), that is Sonic hedgehog protein C-product, was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
Accession# Q15465
Expressed Host HEK293 Cells
Species Human
Predicted N Terminal Cys 198
Molecule Mass The recombinant human SHH( aa 198-462) consists of 276 amino acids and has a predicted molecular mass of 29 kDa. The apparent molecular mass of the protein is approximately 35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
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