| Supplier |
CD BioSciences |
| Product # |
AMA-0669 |
| Pricing |
100 µL, Inquire
|
| Product Category |
Antibodies
|
| Application |
WB, IP
|
| Product Usage Information Dilution |
Western Blotting (1:1000); Immunoprecipitation (1:50)
|
| Reactivity |
Human, Mouse, Rat, Monkey
|
| Gene ID |
5469
|
| UniProt ID |
|
| MW (Target) |
220 kDa
|
| Storage |
Store at -20°C. Do not aliquot the antibody.
|
| Purification |
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His591 of human MED1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
|
| Background |
The mediator complex consists of about 25-30 proteins and is thought to facilitate transcription activation by acting as a molecular bridge between the RNA polymerase II (RNAPII) machinery and transcription factors Mediator is recruited to target genes by transcription factors and plays an essential role in the recruitment and stabilization of the RNAPII transcription complex at promoters, as well as the activation of transcription post RNAPII recruitment The mediator complex also plays an important role in creating ‘chromatin loops’ that occur as a result of interactions between the transcription factor bound at distal enhancers and RNAPII bound at the proximal promoter, and works to sustain proper chromatin architecture during active transcription.
|