hsa-mir-100-3p Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
Detect mature miRNA hsa-mir-100-3p expression normalized by cel-mir-39-3p.
Supplier | Cohesion Biosciences |
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Product # | CPK4070 |
Pricing | 50 rxns USD $300.0, 100 rxns USD $480.0, 200 rxns USD $750.0 |
Reactivity | H |
Applications | Real-time RT-PCR |
Delivery Time | Ship in 3 weeks |
PCR Method | 2-Step qRT-PCR |
Detection Range | Seven orders of magnitude |
Sensitivity | 10 copies of a standard mimics and as little as 25 pg of total RNA |
Sample Types | miRNA, Total RNA |
Platform | ABI 7500 System/ViiA™ 7 System/StepOne™/StepOnePlus™/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480 |
Application | The Real-Time RT-PCR detection and cel-mir-39-3p calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and cel-mir-39-3p primer set for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set. |
Storage/Stability | Shipped at 4°C. Store at -20°C for 6 months. |
Datasheet | http://www.cohesionbio.com/download/CPK4070.pdf |