Phospho-PI3 Kinase Class III (Ser249) Antibody

• Supplier: CD BioSciences
• Product #: AMA-0321
• Product Category: Antibodies
• Application: WB
• Product Usage Information Dilution: Western Blotting (1:1000)
• Reactivity: Human
• Gene ID: 5289
• MW (Target): 100 kDa
• Storage: Store at -20°C. Do not aliquot the antibody.
• Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser249 of human PI3 kinase class III protein. Antibodies are purified by protein A and peptide affinity chromatography.
• Background: Three distinct types of phosphoinositide 3-kinases (PI3K) have been characterized. Unlike other PI3Ks, PI3K class III catalyzes the phosphorylation of phosphatidylinositol at the D3 position, producing phosphatidylinositol-3-phosphate (PIP3) PI3K class III is the mammalian homolog of Vps34, first identified in yeast. PI3K class III interacts with the regular subunit p150, the mammalian homolog of Vps15, which regulates cellular membrane association through myristoylation PIP3 recruits several proteins with FYVE or PX domains to membranes regulating vesicular transport and protein sorting Moreover, PI3K class III has been shown to regulate autophagy, trimeric G-protein signaling, and the mTOR nutrient-sensing pathwayPI3 Kinase Class III/VPS34 is phosphorylated at Ser249 by the autophagy kinase ULK1.