Ascorbic Acid (AA) ELISA Kit

The ELISA kit applies the double-antibody sandwich method to determine the level of Ascorbic Acid (AA)in plant samples. Purified Ascorbic Acid (AA)antibodies are coated onto a microplate to form a solid-phase antibody. To these coated monoclonal antibody wells, Ascorbic Acid (AA)is added, followed by an HRP-conjugated Ascorbic Acid (AA)antibody, forming an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added and turns blue under the catalysis of HRP, and then changes to a final yellow color due to the addition of acid. The color intensity is directly proportional to the amount of Ascorbic Acid (AA)present in the sample. The absorbance (OD value) is measured at 450 nm using an ELISA reader, and the concentration of Ascorbic Acid (AA)in the plant samples is calculated based on a standard curve.

• Supplier: Lifeasible
• Product #: PEK-124
• specs: 96T/48T