| Supplier |
CD BioSciences |
| Product # |
AMA-0289 |
| Pricing |
100 µL, Inquire
|
| Product Category |
Antibodies
|
| Application |
WB, IHC, IF, F
|
| Product Usage Information Dilution |
Western Blotting (1:1000); Immunohistochemistry (1:100-1:400); Immunohistochemistry (1:200-1:800); Flow Cytometry (1:100-1:400)
|
| Reactivity |
Human
|
| Host |
Rabbit
|
| Gene ID |
1029
|
| UniProt ID |
|
| Source/Isotype |
Rabbit IgG
|
| MW (Target) |
14 kDa
|
| Storage |
Store at -20°C. Do not aliquot the antibody.
|
| Purification |
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro126 of human p14 ARF protein.
|
| Background |
Human p14 ARF (p19 ARF in mouse) is a pro-apoptotic cell cycle regulator frequently inactive in human tumors. Basal expression of p14 ARF is low in most cell types, but accumulation of this protein occurs in response to oncogene expression. Increased p14 ARF levels facilitate MDM2 degradation, leading to increased p53 protein levels and subsequent cell cycle arrest and/or apoptosis. While most p14 ARF signaling has traditionally thought to be p53-dependent, more recent reports have described p53-independent p14 ARF signaling leading to cell cycle arrest and apoptosis.
|