EF1汐 Polytronic Thomson Factor Lentivirus without GFP

The 3rd generation polycistronic lentivirus expresses all of the four human transcription factors (hOSNL of Thomson set: Oct4, Sox2, Lin28 and Nanog) within a single expression cassette under the control of EF1汐 promoter. Each of the factor's ORF is separated by a unique 2A peptide (P2A, T2A, and E2A) sequence, which allows for the equivalent expression of all four transcription factors from a single vector. The expression of all factors from a single virus minimizes the number of proviral integrations required for successful reprogramming. This also reduces the risk of insertional mutagenesis effects in the resultant induced pluripotent stem (iPS) cells when compared to those generated using four independent viruses. Also, the virus is provided as concentrated and VSV-G pseudotyped stock capable of transducing both dividing and non-dividing cells. A control GFP lentivirus is included for the easy tracking of gene transduction efficiency in your target cells.

• Supplier: Amerigo Scientific
• Product #: APPL_G414