Cathepsin K substrate

• Supplier: Creative Peptides
• Product #: P75004
• MolecularWeight: 920
• Sequence:
  • One Letter Code: Abz-HPGGPQ-EDDnp
  • Three Letter Code: Abz-His-Pro-Gly-Gly-Pro-Gln-EDDnp
• Storage: -20°C
• Explanation: This FRET peptide can be used to monitor selectively cathepsin K activity in physiological fluids and cell lysates. Abz-HPGGPQ-EDDnp [where Abz represents o-aminobenzoic acid and EDDnp represents N -(2,4-dinitrophenyl)-ethylenediamine], a substrate initially developed for trypanosomal enzymes, is efficiently cleaved at the Gly-Gly bond by cathepsin K. This peptide is resistant to hydrolysis by cathepsins B, F, H, L, S and V, Ex/Em=340 nm/420 nm.
• Reference: Lecaille, F. et al. Biochem. J. 375, 307(2003).