Y-27632, Dihydrochloride Salt (Y27, CAS 129830-38-2), >99%

LC Laboratories' Product Number Y-5301 - Y-27632, Dihydrochloride Salt (Y27, CAS 129830-38-2), >99% - for research use only. Y-27632 is a novel and specific Rho-associated coiled-coil forming protein kinase (ROCK) inhibitor. Narumiya, S., et al. "Use and properties of ROCK-specific inhibitor Y-27632. This research compound is the dihydrochloride salt form of Y-27632. We also offer the free base form; please see Y-27632, Free Base, Cat. No. Y-5399. Y-27632 inhibited ROCK-I (Ki = 0.22 µM) and ROCK-II (Ki = 0.30 µM) by competing with ATP for its binding to the kinase. Y-27632 selectively inhibited Ca2+ sensitization of smooth muscle and blocked its contraction, prevented Rho-induced, p160ROCK-mediated formation of stress fibers in cultured cells, and dramatically lowered hypertension in several hypertensive rat models. Y-27632 inhibited cell transformation mediated by RhoA and its guanine-nucleotide exchange factor (GEF). Y-27632 inhibited both Rho-mediated activation of actomyosin and invasive activity of rat MM1 hepatoma cells. Continuous delivery of Y-27632 largely prevented the dissemination of MM1 cells implanted into the peritoneal cavity of syngeneic rats. Y-27632 blocked chemotactic peptide-induced development of cell polarity and locomotion, and inhibited myosin light chain phosphorylation with similar potency (ED50 = 0.5-1.1 µM). Y-27632 had antinociceptive properties, possibly by inhibition of Rho-kinase. Y-27632 blocked the development of ischemia/reperfusion-induced acute renal failure, possibly by inhibiting myeloperoxidase activity in an early phase after reperfusion. Y-27632 prevented intrahepatic metastasis of human hepatocellular carcinoma. Y-27632 inhibited both of lysophosphatidic acid (LPA)- and fibronectin (FN)-induced migration and morphological change of rat ascites hepatoma (MM1) cells. It impaired LPA- and FN-evoked formation of focal adhesions and actin bundles and suppressed LPA- and FN-induced tyrosine phosphorylation of focal adhesion kinase and paxillin in MM1 cells. This Y-27632 product is the dihydrochloride salt, whose CAS number is 129830-38-2. The CAS number of the free base form is 146986-50-7. Related CAS number: 331752-47-7 for Y-27632 diHCl salt monohydrate. A CAS number previously assigned to Y-27632 diHCl salt monohydrate, namely 203911-23-3, has been deleted by CAS and is no longer in use, and a CAS number previously assigned to Y-27632, Free Base, namely 742043-06-7, has also been deleted by CAS and is also no longer in use. As of October 2013, many other vendors (Adipogen, Affix, AG Scientific, BioVision, Calbiochem/EMD, Cellagen, Enzo, MyBioSource, Reagents Direct, Santa Cruz, Tocris, VDM Biochemicals, etc.) are incorrectly using CAS number 146986-50-7 for the diHCl or diHCl monohydrate salt forms of Y-27632. Chemical Abstracts Service has assigned the CAS number 146986-50-7 to the free base form only.

• Supplier: LC Laboratories
• Product #: Y-5301
• Sku #: Y-5301_250mg