DNA Polymerase High Fidelity (HiFi) Enzyme (with 2.5 mM dNTPs)
DNA Polymerase High Fidelity (HiFi DNA Polymerase) contains T-DNA Polymerase and a proofreading 3'-5' exonuclease. This kit contains two different buffers: HiFi Buffer 1 is optimized for the amplification of genomic DNA, and HiFi Buffer 2 is optimized for the amplification of λDNA, cDNA or plasmid DNA. The extension rate is about 1-2 kb/min. Template-independent ""A"" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into T-vectors. Genomic DNA fragments can be amplified up to 15 kb.
Contents:
Component 250 U 500 U 3 kU HiFi DNA Polymerase250 U 500 U 6 × 500 U 10X HiFi Buffer 1 1.2 ml 1.2 ml 6 × 1.2 ml 10X HiFi Buffer 2 1.2 ml 1.2 ml 6 × 1.2 ml 2.5 mM dNTPs 400 µl 800 µl 6 × 800 µl 10X GC Enhancer 200 µl 400 µl 1 ml 6X DNA Loading Buffer 500 µl 1 ml 2 × 1 ml
Contents:
Component 250 U 500 U 3 kU HiFi DNA Polymerase250 U 500 U 6 × 500 U 10X HiFi Buffer 1 1.2 ml 1.2 ml 6 × 1.2 ml 10X HiFi Buffer 2 1.2 ml 1.2 ml 6 × 1.2 ml 2.5 mM dNTPs 400 µl 800 µl 6 × 800 µl 10X GC Enhancer 200 µl 400 µl 1 ml 6X DNA Loading Buffer 500 µl 1 ml 2 × 1 ml
| Supplier | Abbexa Ltd. |
|---|---|
| Product # | abx071011-250U |
| Pricing | 250 U USD $304.5 |
| Conjugation | Unconjugated |
| Tested Applications | PCR |
| Dry Ice | No |
| Availability | Shipped within 10-20 working days. |
| Note |
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