Dorsomorphin, Free Base (AMPK Inhibitor I, BML-275, BMP Inhibitor I, Compound C, CAS 866405-64-3), >99%

LC Laboratories' Product Number D-3197 - Dorsomorphin, Free Base (AMPK Inhibitor I, BML-275, BMP Inhibitor I, Compound C, CAS 866405-64-3), >99% - for research use only. Dorsomorphin, also known as compound C, is a potent, reversible, ATP-competitive AMP-activated protein kinase (AMPK) inhibitor with Ki of 109 nM in the absence of AMP. This research compound is the free base form of dorsomorphin. We also offer the dihydrochloride salt form; please see Dorsomorphin, Dihydrochloride Salt, Cat. No. D-3456. Inhibition of bone morphogenetic protein (BMP) signaling by dorsomorphin during the early critical stage of differentiation of pluripotent embryonic stem (ES) cells promoted the development of the cardiomyocyte lineage, but at the expense of reduced differentiation of endothelial, smooth muscle, and hematopoietic cells. Dorsomorphin disrupted zebrafish angiogenesis, but not by blocking bone morphogenic protein (BMP) signaling. Dorsomorphin also showed significant off-target effects against the vascular endothelial growth factor (VEGF) pathway.Dorsomorphin reduced phosphorylation levels of SMAD1/5/8 in cells stimulated by BMP2, BMP4, BMP6, and BMP7 selectively, and blocked BMP-, hemojuvelin-, and interleukin 6-mediated hepcidin gene transcription and BMP-induced osteogenic differentiation. It also inhibited hepcidin transcription in iron-stimulated zebrafish and induced hyperferremia in iron-replete mice. The addition of dorsomorphin to the culture medium before treatment with differentiation inducers reduced lipid accumulation in 3T3-L1 cells. Dorsomorphin treatment of MCF7 breast carcinoma cells led to Bax redistribution from the cytoplasm to mitochondria and caused apoptosis at least partly by ceramide upregulation, and probably not by AMPK inhibition. Insulin did not affect glucose transport in control cells but increased glucose uptake for serum-starved cells that was preventable by dorsomorphin. Dorsomorphin inhibited the growth of four colorectal cancer cell lines, caused G2/M arrest, and induced apoptotic or autophagic death. This dorsomorphin product is the free base form, whose CAS number is 866405-64-3. The CAS number of the dihydrochloride salt form is 1219168-18-9.

• Supplier: LC Laboratories
• Product #: D-3197
• Sku #: D-3197_5mg