| Supplier |
Ace Therapeutics |
| Product # |
NSC1217 |
| Pricing |
Inquiry
|
| Product Size |
20 µg
|
| Purity |
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
|
| Product Type |
Proteins
|
| Endotoxin |
< 10 EU per mg protein.
|
| Activity |
- 1.iPSC-derived human vascular organoids (Day 7) were cultured with FGF2, VEGFA, EGF. Red arrows represent vascular organoids. Image taken at 10x magnification.(Routinely tested)
- 2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 2-10 ng/mL.
- 3. Labeled biotin to VEGF165 Protein, Human / Cynomolgus, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of VEGF165 Protein, Human / Cynomolgus, Recombinant, Biotinylated bound to Avastin was 0.1 nM.
|
| Protein Construction |
A DNA sequence encoding the human / cynomolgus VEGF165 isoform (P15692-4) (Met1-Arg191) was expressed. Human and Cynomolgus VEGF165 sequences are identical.
|
| Accession# |
P15692-4
|
| Expressed Host |
HEK293 Cells
|
| Species |
Human, Cynomolgus
|
| Predicted N Terminal |
Ala 27
|
| Molecule Mass |
The recombinant human / cynomolgus VEGF165 consists of 165 amino acids and predicts a molecular mass of 19.2 KDa. It migrates as an approximately 22 kDa and 24 kDa band in SDS-PAGE under reducing conditions.
|
| Formulation |
Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
|
| Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|
| Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Reconstitution |
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
|