Reporter Gene mRNA (5moU) -for Transfection Assay

Reporter genes are commonly used in cell biology research.
OZ Biosciences offers reporter mRNAs thatcan be used as controls to study transfection and expression in mammalian cells using a variety of assays.They mimic fully processed mature mRNA.
These mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). They contain a poly(A) tail at the 3’ end and are also optimized to yield improved stability and performance. They are modified with 5-methoxyuridine (5moU replaces U) to reduce innate immune responses.
We also offer unmodified mRNAs --#MRNA8 (mCherry), #MRNA9 (Tomato), #MRNA15 (GFP), #MRNA16 (F-Luc).
We recommend using ourtransfection reagentsRmesFectorRmesFect Stemin order to reach high transfection efficiency and optimum protein expression level.
mRNA transfection provides several advantages :
No need of nuclear uptake - protein expression directly in cytoplasm
Faster protein expression than DNA transfection
No genomic integration
Protein expression directly related to mRNA quantity
Perfect for transfecting slowing or non-dividing cells
Protein expression in a total promoter-independent manner
Transient transfection: mRNA based expression of proteins sustains for a limited time
GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.
F-Luc mRNA has been designed to produce high expression level of FireFly Luciferase. It is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
Tomato mRNA has been designed to produce high expression level of Orange Fluorescent Protein. The detection can be done by fluorescent or confocal microscopy. The produced Tomato has an excitation maximum at 551-557 nm and emission maximum at 579-583 nm. Tomato expression level can also be monitored by fluorescence-activated cell sorter analysis (FACS).
mCherry mRNAencodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found inDiscosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is photostable and resistant to photobleaching.
β-galactosidase (β-gal) mRNA, codes fora protein product of the bacterial LacZ gene.β-gal catalyzes the conversion ofβ-galactosides into monosaccharides. It is a common marker gene used to assess transfection efficiency.
Size: 20 µg,100µg and 1 mg

• Supplier: Amerigo Scientific
• Product #: MRNA10-20