Phospho-Gab1 (Tyr659) Antibody

• Supplier: CD BioSciences
• Product #: AMA-0323
• Product Category: Antibodies
• Application: WB, IP
• Product Usage Information Dilution: Western Blotting (1:1000); Immunoprecipitation (1:50)
• Reactivity: Human
• Gene ID: 2549
• MW (Target): 110 kDa
• Storage: Store at -20°C. Do not aliquot the antibody.
• Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr659 of human Gab1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
• Background: The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold Multiple tyrosine phosphorylation sites of Gab1 protein have been identified Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2.