EGF Protein, Mouse, Recombinant

• Supplier: Ace Therapeutics
• Product #: NSC881
• Product Size: 100 µg
• Purity: > 95 % as determined by SDS-PAGE
• Product Type: Proteins
• Synonyms: AI790464 Protein, Mouse
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Activity: 1.Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1-0.6 ng/mL.2.Human intestinal cancer organoids were cultured with RSPO1(Cat#11083-HNAS), EGF(Cat#50482-MNCH), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.3.Human colorectum organoids were cultured with RSPO1(Cat#11083-HNAS), EGF(Cat#50482-MNCH), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.4.Human lung cancer organoids were cultured with FGF2(Cat#10014-HNAE), FGF4(Cat#16043-HNAE), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.5.Human lung organoids were cultured with FGF2(Cat#10014-HNAE), FGF4(Cat#16043-HNAE), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.6.Human cholangiocarcinomas organoids were cultured with FGF2(Cat#10014-HNAE), HGF(Cat#10463-HNAS), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.7.Human liver cancer organoids were cultured with FGF2(Cat#10014-HNAE), HGF(Cat#10463-HNAS), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), TGFB1(Cat#10804-HNAC), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.8.Human kidney cancer organoids were cultured with FGF2(Cat#10014-HNAE), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.9.Human kidney organoids were cultured with FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS), HGF(Cat#10463-HNAS), FGF4(Cat#16043-HNAE). (Routinely tested). Data provided by D1 Medical Technology.10.Human gastric cancer organoids were cultured with EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.11.Human stomach organoids organoids were cultured with EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.12.Human breast cancer organoids were cultured with FGF7(Cat#10210-H07E), RSPO1(Cat#11083-HNAS), IGF1(Cat#10598-HNAE), EGF(Cat#50482-MNCH), NRG1 Beta 1(Cat#11609-H01H), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.13.Human ovarian organoids were cultured with IGF1(Cat#10598-HNAE), NRG1 Beta 1(Cat#11609-H01H), RSPO1(Cat#11083-HNAS), EGF(Cat#50482-MNCH), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.14.Human small intestinal organoids were cultured with IL22(Cat#13059-HNAE), FGF10(Cat#10573-HNAE), EGF(Cat#50482-MNCH), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.
• Protein Construction: A DNA sequence encoding the mouse EGF (P01132) (Asn977-Arg1029) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
• Accession#: P01132
• Expressed Host: HEK293 Cells
• Species: Mouse
• Predicted N Terminal: Gly
• Molecule Mass: The recombinant mouse EGF consists of 55 amino acids and predicts a molecular mass of 6.2 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Stability & Storage: Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.