hsa-let-7a Real-Time RT-PCR Detection and U6 Calibration Kit
Detect mature miRNA hsa-let-7a expression normalized by U6 snRNA.
| Supplier | Cohesion Biosciences |
|---|---|
| Product # | CPK2001 |
| Pricing | 50 rxns USD $300.0, 100 rxns USD $480.0, 200 rxns USD $750.0 |
| Reactivity | H |
| Applications | Real-time RT-PCR |
| Delivery Time | Ship in 3 weeks |
| PCR Method | 2-Step qRT-PCR |
| Detection Range | Seven orders of magnitude |
| Sensitivity | 10 copies of a standard mimics and as little as 25 pg of total RNA |
| Sample Types | miRNA, Total RNA |
| Platform | ABI 7500 System/ViiA™ 7 System/StepOne™/StepOnePlus™/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480 |
| Application | The Real-Time RT-PCR detection and U6 calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and U6 primer set as housekeeping gene for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set. |
| Storage/Stability | Shipped at 4°C. Store at -20°C for 6 months. |
| Datasheet | http://www.cohesionbio.com/download/CPK2001.pdf |