DNA Polymerase High Fidelity (HiFi) Enzyme
DNA Polymerase High Fidelity (HiFi DNA Polymerase) contains T-DNA Polymerase and a proofreading 3'-5' exonuclease. This kit contains two different buffers: HiFi Buffer 1 is optimized for the amplification of genomic DNA, and HiFi Buffer 2 is optimized for the amplification of λDNA, cDNA or plasmid DNA. The extension rate is about 1-2 kb/min. Template-independent ""A"" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into T-vectors. Genomic DNA fragments can be amplified up to 15 kb. This kit does not contain 2.5 mM dNTPs.
Contents:
Component 250 U 500 U 3 kU HiFi DNA Polymerase250 U 500 U 6 × 500 U 10X HiFi Buffer 1 1.2 ml 1.2 ml 6 × 1.2 ml 10X HiFi Buffer 2 1.2 ml 1.2 ml 6 × 1.2 ml 10X GC Enhancer 200 µl 400 µl 1 ml 6X DNA Loading Buffer 500 µl 1 ml 2 × 1 ml
Contents:
Component 250 U 500 U 3 kU HiFi DNA Polymerase250 U 500 U 6 × 500 U 10X HiFi Buffer 1 1.2 ml 1.2 ml 6 × 1.2 ml 10X HiFi Buffer 2 1.2 ml 1.2 ml 6 × 1.2 ml 10X GC Enhancer 200 µl 400 µl 1 ml 6X DNA Loading Buffer 500 µl 1 ml 2 × 1 ml
Supplier | Abbexa Ltd. |
---|---|
Product # | abx071010-250U |
Pricing | 250 U USD $304.5 |
Conjugation | Unconjugated |
Tested Applications | PCR |
Dry Ice | No |
Availability | Shipped within 10-20 working days. |
Note |
|