Phospho-IRS-1 (Ser318) (D51C3) Rabbit Antibody

Supplier CD BioSciences
Product # AMA-0010
Pricing 100 μL, Inquire
Product Category Antibodies
Application WB, IP
Product Usage Information Dilution Western Blotting (1:1000); Immunoprecipitation (1:50)
Reactivity Human, Mouse
Host Rabbit
Gene ID 3667
UniProt ID
Source/Isotype Rabbit IgG
MW (Target) 180 kDa
Storage Store at -20°C.
Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser318 of mouse IRS-1 protein.
Background Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.PKC phosphorylates mouse IRS-1 at Ser318 (human Ser323) by insulin receptor activation or by other stimulation such as TPA, IL-6, retinoic acid treatment . The phosphorylation at Ser318 acts as a negative feedback signal to down regulates of insulin effect.
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