| Supplier |
Ace Therapeutics |
| Product # |
NSC2535 |
| Pricing |
Inquiry
|
| Product Size |
100 µg
|
| Purity |
> 95 % as determined by SDS-PAGE
|
| Product Type |
Proteins
|
| Synonyms |
Noggin Protein, Human; SYM1 Protein, Human; SYNS1 Protein, Human
|
| Endotoxin |
< 1.0 EU per μg of the protein as determined by the LAL method
|
| Activity |
Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.1-0.6 μg/mL in the presence of 25 ng/mL of hBMP4.
|
| Protein Construction |
A DNA sequence encoding the human Noggin precursor (NP_005441.1) (Met 1-Cys 232) was fused with the Fc region of human IgG1 at the C-terminus.
|
| Accession# |
NP_005441.1
|
| Expressed Host |
HEK293 Cells
|
| Species |
Human
|
| Predicted N Terminal |
Gln 28
|
| Molecule Mass |
The recombinant human Noggin/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. Each monomer comprises 443 amino acids and has a predicted molecular mass of 49.8 kDa. As a result of glycosylation, the apparent molecular mass of rhNoggin/Fc monomer is approximately 58-62 kDa in SDS-PAGE under reducing conditions.
|
| Formulation |
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
|
| Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|
| Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Reconstitution |
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
|