O-phospho-L-serine-tRNA ligase

In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 (cysteine-tRNA ligase) for the direct Cys-tRNACys formation, Cys-tRNACys is produced by an indirect pathway, in which EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) ligates O-phosphoserine to tRNACys, and EC 2.5.1.73 (O-phospho-L-seryl-tRNA: Cys-tRNA synthase) converts the produced O-phospho-L-seryl-tRNACys to Cys-tRNACys. The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism. Methanosarcina mazei can use both pathways, the direct route using EC 6.1.1.16 (cysteine-tRNA ligase) and the indirect pathway with EC 6.1.1.27 and EC 2.5.1.73 (O-phospho-L-seryl-tRNA: Cys-tRNA synthase).

• Supplier: Creative Enzymes
• Product #: EXWM-5658