gellan tetrasaccharide unsaturated glucuronyl hydrolase

The enzyme releases 4-deoxy-4(5)-unsaturated D-glucuronic acid from oligosaccharides produced by polysaccharide lyases, e.g. the tetrasaccharide β-D-4-deoxy-Δ4-GlcAp-(1→4)-β-D-Glcp-(1→4)-α-L-Rhap-(1→3)-β-D-Glcp produced by EC 4.2.2.25, gellan lyase. The enzyme can also hydrolyse unsaturated chondroitin and hyaluronate disaccharides (β-D-4-deoxy-Δ4-GlcAp-(1→3)-β-D-GalNAc, β-D-4-deoxy-Δ4-GlcAp-(1→3)-β-D-GalNAc6S, β-D-4-deoxy-Δ4-GlcAp2S-(1→3)-β-D-GalNAc, β-D-4-deoxy-Δ4-GlcAp-(1→3)-β-D-GlcNAc), preferring the unsulfated disaccharides to the sulfated disaccharides.

• Supplier: Creative Enzymes
• Product #: EXWM-3862