Phospho-MDM2 (Ser166) Antibody
| Supplier | CD BioSciences |
|---|---|
| Product # | AMA-0592 |
| Pricing | , Inquire |
| Product Category | Antibodies |
| Application | WB |
| Product Usage Information Dilution | Western Blotting (1:1000) |
| Reactivity | Human, Mouse, Rat |
| Gene ID | 4193 |
| UniProt ID | |
| MW (Target) | 90 kDa |
| Storage | Store at -20°C. Do not aliquot the antibody. |
| Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser166 of human MDM2. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. |