S-adenosylmethionine (SAM) ELISA kit for cell culture supernatant or other samples

Methionine is an amino acid with sulfur methyl group, which is converted into S-adenosylmethionine (SAM) with ATP by methionine adenosyltransferase (EC 2.5.1.6). SAM is the sole methyl group donor for over 50 kinds of biologically active substances such as DNA, RNA, protein, phospholipids, hormones and neuro-transmitters, etc. After methyl group is transferred away from SAM under a mehtyltransferase, S­adenosylhomocysteine is formed, which is further metabolized into homocysteine (Hcy) after adenosine is removed. The homocysteine is metabolized to methionine through N5-methyl tetrahydrofolate methyltransferase (EC1.1.1.68) and coenzyme vitamin B 12 catalytic pathway accepting methyl from N5-methyltetrahydrofolate. This is methionine cycle.
Methylation index is defined as the ratio of SAM and SAH. Methylation index is a better marker for methylation status and methylation capability.
SAM is used as medicines or nutritional supplements for treating depression, liver diseases, arthritis and joint pain, etc.

• Supplier: Amerigo Scientific
• Product #: IK00204