hsa-mir-221-5p RT-PCR Primer Set
Stem-loop RT primer and PCR primer set to detect mature miRNA hsa-mir-221-5p expression.
| Supplier | Cohesion Biosciences |
|---|---|
| Product # | CPK3159 |
| Pricing | 1 nmol USD $100.0 |
| Source | Synthetic |
| Reactivity | H |
| Applications | RT-PCR, Real-time RT-PCR |
| Delivery Time | Ship in 3 weeks |
| PCR Method | 2-Step qRT-PCR |
| Detection Range | Seven orders of magnitude |
| Sensitivity | 10 copies of a standard mimics and as little as 25 pg of total RNA |
| Sample Types | miRNA, Total RNA |
| Platform | ABI 7500 System/ViiA™ 7 System/StepOne™/StepOnePlus™/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480 |
| Application | The miRNAs RT-PCR primer set contains a stem-loop RT primer and a pair of specific PCR primer set. Combined with the custom's RT and PCR reagent. It provides a sensitive and specific method of real-time PCR for quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set. |
| Storage/Stability | Shipped at 4°C. Store at -20°C for 2 years. |
| Datasheet | http://www.cohesionbio.com/download/CPK3159.pdf |