Recombinant Human Noggin/NOG Protein, HPLC-verified

Supplier Ace Therapeutics
Product # NSC2533
Pricing Inquiry
Product Size 20 µg
Purity ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Product Type Proteins
Synonyms Noggin Protein, Human; SYM1 Protein, Human; SYNS1 Protein, Human
Endotoxin < 10 EU per mg protein.
Activity Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells.The ED50 for this effect is 10-80 ng/mL.
Protein Construction A DNA sequence encoding the human NOG (NP_005441.1) (Met1-Cys232) was expressed.
Accession# NP_005441.1
Expressed Host HEK293 Cells
Species Human
Predicted N Terminal Gln 28
Molecule Mass The recombinant human NOG consists of 205 amino acids and predicts a molecular mass of 23.1 kDa. As a result of glycosylation, the human NOG migrates as approximately 30 kDa band in SDS-PAGE under reducing conditions.
Formulation Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
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