hsa-mir-299 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

Detect mature miRNA hsa-mir-299 expression normalized by cel-mir-39-3p.

• Supplier: Cohesion Biosciences
• Product #: CPK4167
• Reactivity: H
• Applications: Real-time RT-PCR
• Delivery Time: Ship in 3 weeks
• PCR Method: 2-Step qRT-PCR
• Detection Range: Seven orders of magnitude
• Sensitivity: 10 copies of a standard mimics and as little as 25 pg of total RNA
• Sample Types: miRNA, Total RNA
• Platform: ABI 7500 System/ViiA™ 7 System/StepOne™/StepOnePlus™/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480
• Application: The Real-Time RT-PCR detection and cel-mir-39-3p calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and cel-mir-39-3p primer set for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.
• Storage/Stability: Shipped at 4°C. Store at -20°C for 6 months.
• Datasheet: http://www.cohesionbio.com/download/CPK4167.pdf