| Supplier |
Ace Therapeutics |
| Product # |
NAE008 |
| Pricing |
Inquiry
|
| Product Size |
50 µg
|
| Purity |
≥ 90 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
|
| Product Type |
Proteins
|
| Synonyms |
AADC Protein, Human
|
| Endotoxin |
< 1.0 EU per μg of the protein as determined by the LAL method
|
| Activity |
Measured by its ability to convert the substrate 3, 4-dihydroxy L-phenylalanine (L-Dopa) to 3, 4-dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. The specific activity is >1000 pmoles/min/μg.
|
| Protein Construction |
A DNA sequence encoding the full length of human DDC (NP_000781.1) (Met 1-Glu 480) was expressed with a polyhistidine tag at the C-terminus.
|
| Accession# |
NP_000781.1
|
| Expressed Host |
Baculovirus-Insect Cells
|
| Species |
Human
|
| Predicted N Terminal |
Met
|
| Molecule Mass |
The recombinant human DDC consists of 490 amino acids and predicts a molecular mass of 55 kDa. It migrates as an approximately 48 kDa protein in SDS-PAGE under reducing conditions.
|
| Formulation |
Supplied as sterile 50mM Tris, 100mM NaCl, pH 8, 10% glycerol. Please contact us for any concerns or special requirements.. Please refer to the specific buffer information in the hard copy of CoA.
|
| Shipping |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
|
| Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Reconstitution |
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
|