| Supplier |
CD BioSciences |
| Product # |
AMA-0381 |
| Pricing |
20 µL/100 µL/300 µL, Inquire
|
| Product Category |
Antibodies
|
| Application |
WB, IF, F
|
| Product Usage Information Dilution |
Western Blotting (1:1000); Immunofluorescence (1:50); Flow Cytometry (1:50)
|
| Reactivity |
Human
|
| Gene ID |
7535
|
| UniProt ID |
|
| MW (Target) |
70 kDa
|
| Storage |
Store at -20°C. Do not aliquot the antibody.
|
| Purification |
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70. Antibodies are purified by protein A and peptide affinity chromatography.
|
| Background |
The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemiaPhosphorylation of Tyr319 is required for the assembly of a Zap-70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1.
|