TopTaqDNA Polymerase Enzyme (with 2.5 mM dNTPs)
TopTaq DNA Polymerase contains three binding proteins: one binding protein binds to double-stranded DNA template, preventing polymerase activity at room temperature, and the other two binding proteins bind primers, preventing primer-dimer formation. Together, these binding proteins reduce non-specific amplification. The blocking proteins are released from primers and templates during the initial denaturation. This double-blocking method has higher efficiency and specificity than antibody-based or chemically-modified hot start PCR. Template-independent ""A"" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into T-vectors. This kit does not use Taq antibodies, reducing the risk of mammalian DNA contamination, and this kit does not use chemical modification, so a long denaturing step is not required. Genomic DNA fragments can be amplified up to 15 kb.
Contents:
Component 250 U 500 U 3 kU TopTaq DNA Polymerase250 U 500 U 6 × 500 U 10X TopTaq Buffer 1.2 ml 2 × 1.2 ml 12 × 1.2 ml 2.5 mM dNTPs 800 µl 2 × 800 µl 8 × 1.2 ml 10X GC Enhancer 200 µl 400 µl 1 ml 6X DNA Loading Buffer 500 µl 1 ml 2 × 1 ml
Contents:
Component 250 U 500 U 3 kU TopTaq DNA Polymerase250 U 500 U 6 × 500 U 10X TopTaq Buffer 1.2 ml 2 × 1.2 ml 12 × 1.2 ml 2.5 mM dNTPs 800 µl 2 × 800 µl 8 × 1.2 ml 10X GC Enhancer 200 µl 400 µl 1 ml 6X DNA Loading Buffer 500 µl 1 ml 2 × 1 ml
| Supplier | Abbexa Ltd. |
|---|---|
| Product # | abx071015-500U |
| Pricing | 500 U USD $435.0 |
| Conjugation | Unconjugated |
| Tested Applications | SDS-PAGE |
| Dry Ice | No |
| Availability | Shipped within 10-20 working days. |
| Note |
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