Phospho-(Ser) 14-3-3 Binding Motif Antibody

• Supplier: CD BioSciences
• Product #: AMA-0242
• Product Category: Antibodies
• Application: WB, IP, IHC
• Product Usage Information Dilution: Western Blotting (1:1000); Immunoprecipitation (1:50); Immunohistochemistry (1:100)
• Reactivity: All Species
• Host: Rabbit
• Storage: Store at -20°C. Do not aliquot the antibody.
• Purification: Polyclonal antibodies are produced by immunizing animals with synthetic phospho-(Ser) 14-3-3-binding-motif containing peptides . Antibodies are purified by protein A and peptide affinity chromatography.
• Background: The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control. Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions . Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins. Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25C, FKHRL1, PKC and c-Raf. Phospho-(Ser) 14-3-3 Binding Motif Polyclonal and (4E2) Monoclonal Antibodies provide powerful tools for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.