Proteases Substrate, Fluorogenic

• Supplier: Creative Peptides
• Product #: X21157
• Sequence:
  • One Letter Code: (Z-R)2Rh110 • 2HCl
  • Three Letter Code: (Z-Arg)2Rh110•2HCl
• Storage: -20°C desiccated and protected from light
• Explanation: This is a fluorescent generic fluorogenic substrate for proteases such as trypsin, cathepsin B and L, Abs/Em=501/527. The colorless (Z-Arg)2Rh110 is hydrolyzed to the highly fluorescent Rhodamine 110, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments.
• Solubility: DMSO
• Reference:
  • 1. Halangk W, et al. (1997). Trypsinogen activation in rat pancreatic acinar cells hyperstimulated by caerulein. Biochim Biophys Acta 1362, 243-51,
  • 2. Banati RB, et al. (1993). Detection of lysosomal cysteine proteinases in microglia: flow cytometric measurement and histochemical localization of cathepsin B and L. Glia 7, 183-91,
  • 3. Rothe G, et al. (1992). Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54.