(R)-specific secondary-alkylsulfatase

The enzyme from Rhodococcus ruber is involved in the biodegradation of alkyl sulfate esters used as detergents and released into the environment. The prefered substrates are linear secondary-alkyl sulfate esters, particularly octan-2-yl, octan-3-yl, and octan-4-yl sulfates. The enzyme from Pseudomonas sp. DSM6611 utilizes a range of secondary-alkyl sulfate esters bearing aromatic, olefinic and acetylenic moieties. Perfect enantioselectivities are obtained for substrates bearing groups of different size adjacent to the sulfate moiety. The enzymic hydrolysis proceeds through inversion of the configuration at the stereogenic carbon atom. The enzyme contains a Zn2+ ion.

• Supplier: Creative Enzymes
• Product #: EXWM-3752