GV3101 (pSoup-p19) Chemically Competent Cell
The genotype of GV3101 (pSoup-p19) Chemically Competent Cell is C58 (rif R) Ti pMP90 (pTiC58DT-DNA) (gent R) Nopaline (pSoup-p19-tet R). The p19 protein is derived from tomato bush dwarf virus, which can inhibit the RNA silencing effect of foreign genes, improve the stability of heterologous gene transcripts. Background of GV3101 (pSoup) strain is C58. It contains rifampicin resistant gene (rif) in nuclear genes as screening label. GV3101 carries nopaline type Ti plasmid pMP90 (pTiC58DT-DNA) to facilitate transformation. Ti plasmid pMP90 (pTiC58DT-DNA) contains vir gene, which is essential for insertion of T-DNA into plant genome. Ti plasmid pMP90 (pTiC58DT-DNA) is disabled to transfer its own T-DNA but enabled to transfer foreign binary vector T-DNA. pMP90 (pTiC58DT-DNA) Ti plasmid contains gentamicin resistance gene.The pSoup-p19 plasmid helps the pGreen, 62SK, pGs2 series plasmids to replicate in Agrobacterium.
Supplier | Lifeasible |
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Product # | ACC-102 |
Pricing | 10 tubes; 20 tubes; 50 tubes; 100 tubes, USD $388; $468; $738; $1,148 |
specs | 100μL/tube |