| Supplier |
CD BioSciences |
| Product # |
AMA-0625 |
| Pricing |
100 µL, Inquire
|
| Product Category |
Antibodies
|
| Application |
WB, IP
|
| Product Usage Information Dilution |
Western Blotting (1:1000); Immunoprecipitation (1:100)
|
| Reactivity |
Human, Mouse, Rat, Monkey
|
| Host |
Rabbit
|
| Gene ID |
3093
|
| UniProt ID |
|
| Source/Isotype |
Rabbit IgG
|
| MW (Target) |
25 kDa
|
| Storage |
Store at -20°C. Do not aliquot the antibody.
|
| Purification |
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu161 of human HIP2 protein.
|
| Background |
Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3Huntingtin-interacting protein-2 (HIP2), also known as E2-25K, is a member of the E2 protein family that is highly expressed in the brain and catalyzes multiubiquitin chain synthesis via Lys48 of ubiquitin E2-25K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-β, huntingtin, and MHC-heavy chain proteins Recent studies have also implicated HIP2 in the control of apoptosis and cell-cycle progression through its ability to regulate the stability of Smac/Diablo and cyclin B1.
|