CD47 (D3O7P) Rabbit Antibody (BSA and Azide Free)

Supplier CD BioSciences
Product # AMA-0351
Pricing 100 µg, Inquire
Product Category Antibodies
Application WB, IHC
Reactivity Human
Host Rabbit
Gene ID 961
UniProt ID
Source/Isotype Rabbit IgG
MW (Target) 45-50 kDa
Storage Store at -20°C. Do not aliquot the antibody.
Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu72 of human CD47 protein.
Background CD47 is a five-pass transmembrane protein expressed on all normal cells. It binds to the SIRPa that is expressed on myeloid cells including macrophages, and neuronal cells in the central nervous system. Binding of CD47 to SIRPα promotes phosphorylation of tyrosine residues in the immunoreceptor tyrosine-based inhibitory motifs (ITIM) within theSIRPα cytoplasmic tail, inhibiting macrophage phagocytosis towards CD47-expressing cells. In this way, CD47 serves as "don't eat me" signal or a marker of "self", functioning as an innate immune checkpoint. Additionally, CD47 was reported to modulate lymphocyte cell activation and proliferation CD47 is over-expressed in many types of cancer. The expression level of CD47 on cancer cells is negatively associated with the response to therapies, and low expression on tumor cells is associated with a better prognosis and survival. Reagents that can block CD47-SIRPα interaction are being actively pursued for therapeutic applications In addition to SIRPα, other proteins have been reported to bind to CD47. Thrombospondin 1 (TSP1) competes with SIRPα to bind to CD47 in the extracellular region and activates signaling pathways downstream CD47 CD47 can laterally associate with VEGFR2, FAS, and certain integrins in different contexts, and influences their downstream signaling CD47 can be shed from the cell surface by proteolytic cleavage. In addition, CD47 is present on extracellular vesicles including exosomes, suggesting additional extracellular signaling potential.
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