PureIT ExoZAP PCR CleanUp

One-step PCR Clean-up for optimal sequencing results. Clean-up results in 5 minutes.

FEATURES
One-step PCR product clean up
Fast 5 min protocol
No Sample Loss
No need for spin columns or magnetic beads
Treatment improves downstream applications such as DNA sequencing and SNP analysis
Ideal for NGS library preparation protocols

PureIT ExoZAP PCR CleanUp consists of a balanced combination of a heat labile Exonuclease (HL-ExoI) I and a recombinant Shrimp Alkaline Phosphatase (rSAP).
Treatment of amplified PCR products with PureIT ExoZAP PCR CleanUp, removes residual primers, single-stranded DNA and inactivates excess dNTPs by dephosphorylation. Add PureIT ExoZAP PCR CleanUp directly to the reaction containing the amplified PCR product. After treatment at 37 °C for minimum 2 minutes, PureIT ExoZAP PCR CleanUp is completely inactivated by heating at 80 °C for minimum 3 minutes.

PROTOCOL
This protocol serves as a guideline for clean-up of 5 μl PCR product using PureIT ExoZAP PCR CleanUp*.
Take out PureIT ExoZAP PCR CleanUp from the -20 °C freezer.
Keep PureIT ExoZAP PCR CleanUp on ice at all times.
Add 2 μl PureIT ExoZAP PCR CleanUp** to 5 μl of amplified PCR product.
Mix well and spin down.
Incubate the reaction at 37 °C for 2 - 5 minutes to degrade remaining primers, single-stranded DNA and to inactivate excess nucleotides by dephosphorylation.
Incubate at 80 °C to completely inactivate PureIT ExoZAP PCR CleanUp for 3 - 10 minutes.
The cleaned up PCR product can now be used for downstream applications such DNA sequencing, primer extension experiment or SNP analysis.
After treatment the PCR products can be stored at -20 °C
*If treating PCR product of higher volume, then increase proportionally the amount of PureIT ExoZAP PCR CleanUp.
**PureIT ExoZAP PCR CleanUp works in PCR buffers.

• Supplier: Amerigo Scientific
• Product #: A620601