-
No products found
because this supplier's products are not listed.
Helene Sertznig, et al.,
bioRxiv - Microbiology 2021
Quote:
... Empigen zwitterionic detergent (Sigma) was added to the samples for inactivation of HIV-1 and incubated for 30 min at 56 °C ...
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No products found
because this supplier's products are not listed.
Haidai Hu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 0.5% zwitterionic detergent CHAPSO (Anatrace) was added to the purified sample to a final concentration of 0.0125% before cryo-EM grid preparation ...
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No products found
because this supplier's products are not listed.
Briana Zellner, et al.,
bioRxiv - Microbiology 2021
Quote:
... 3-cholamidopropyl dimethylammonio 1-propanesulfonate (CHAPS; zwitterionic; Thermo Scientific). In addition ...
-
No products found
because this supplier's products are not listed.
Constanze Depp, et al.,
bioRxiv - Neuroscience 2021
Quote:
... detergent compatible protein assays (Biorad) were carried out in duplicates ...
-
No products found
because this supplier's products are not listed.
Hannah Donnelly, et al.,
bioRxiv - Bioengineering 2024
Quote:
... the zwitterionic ZICpHILLIC column (150 mm x 4.6 mm; 3.5 µm, Merck) and the reversed phase ACE C18-AR column (150 mm x 4.6 mm ...
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No products found
because this supplier's products are not listed.
Lucía Nitsch-Velásquez,
bioRxiv - Microbiology 2020
Quote:
... alconox (anionic detergent, Sigma–Alconox Inc.), saponin (non–ionic surfactant ...
-
No products found
because this supplier's products are not listed.
Fouzia Bano, et al.,
bioRxiv - Microbiology 2024
Quote:
... were cleaned using 7x detergent (MP Biomedicals, CA) and milli-Q water with 1:6 (volume ...
-
No products found
because this supplier's products are not listed.
Matthieu Sanial, et al.,
bioRxiv - Biochemistry 2024
Quote:
Relative quantitations were carried out with a detergent-compatible Bradford reagent (Abcam, #ab119216), and samples were then incubated for 5 min ...
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No products found
because this supplier's products are not listed.
P. Mühlenbrock, et al.,
bioRxiv - Biophysics 2020
Quote:
... detergent was removed by size exclusion chromatography (illustra NAP-10 G25 column; GE Healthcare) in buffer A ...
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No products found
because this supplier's products are not listed.
Tyler D.R. Vance, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... a detergent screen (Hampton Research) was used to identify conditions that yielded strongly diffracting crystals ...
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No products found
because this supplier's products are not listed.
Jan T. Czerminski, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... and detergent extracted in 0.5% Triton X-100 (Roche) for 3min.
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No products found
because this supplier's products are not listed.
Liliana S. O. Silva, et al.,
bioRxiv - Biochemistry 2021
Quote:
... XRL hanging drop 24-well MD3-11 plates (Molecular Dimensions) were used for protein crystallization ...
-
No products found
because this supplier's products are not listed.
Mariah R. Pierce, et al.,
bioRxiv - Biochemistry 2023
Quote:
Detergents were ordered as part of a solubilization screening kit (Popular Detergent Kit; 850561P-1EA, Avanti Polar Lipids) or individually for Fos-Choline-16 (F316 ...
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No products found
because this supplier's products are not listed.
Laura M Bartos, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Detergent removal and subsequent digestion with 0.2 µg LysC and 0.2 µg trypsin (Promega, Walldorf, Germany) was performed using the single-pot ...
-
No products found
because this supplier's products are not listed.
Juanita C. Limas, et al.,
bioRxiv - Cell Biology 2021
Quote:
... & detergent (Active Motif, cat# 37517). Lysates were then incubated with beads that had been pre-incubated with antibody in cold blocking buffer (1X PBS + protease/phosphatase inhibitors + ATP ...
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No products found
because this supplier's products are not listed.
Nicky Craig, et al.,
bioRxiv - Microbiology 2021
Quote:
... each detergent lysis buffer was supplemented with 0.1AU/ml proteinase K (Qiagen) and 0.83mM final concentration EDTA (Sigma ...
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No products found
because this supplier's products are not listed.
Prasath Paramasivam, et al.,
bioRxiv - Cell Biology 2021
Quote:
... All detergents were added to cells using the liquid handling robot Biomek FX (Beckman Coulter) equipped with a 384 disposable tip head ...
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No products found
because this supplier's products are not listed.
Steve Horvath, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Tryptic glycopeptides were enriched on Chromabond® zwitterionic HILIC adsorbent (Macherey-Nagel GmbH & Co., Düren, Germany) in a high-throughput manner ...
-
No products found
because this supplier's products are not listed.
Seoyoon Kim, et al.,
bioRxiv - Biophysics 2023
Quote:
... 1% of DDM detergent (GoldBio) was added to the lysed cell and the cells was incubated for 1 h at 4°C to extract the membrane protein ...
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No products found
because this supplier's products are not listed.
Dylan Fitzmaurice, et al.,
bioRxiv - Biophysics 2024
Quote:
... and detergent-lysed (LB+ 3M sorbitol+ N-lauroylsarcosine sodium salt) ...
-
No products found
because this supplier's products are not listed.
Tong Su, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Protein samples were purified using HiPPR detergent removal resin (PI88305, VWR International) and dried completely by Speedvac (Savant ...
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No products found
because this supplier's products are not listed.
Fadi Jacob, et al.,
bioRxiv - Neuroscience 2020
Quote:
... sections were washed with TBS to remove detergent and incubated with TrueBlack reagent (Biotium) diluted 1:20 in 70% ethanol for 1 minute to block autofluorescence ...
-
No products found
because this supplier's products are not listed.
Abner Gonzalez, et al.,
bioRxiv - Biophysics 2023
Quote:
... 2 mM β-mercaptoethanol and 0.003125% [w/v] NP-40 (#S226, Biovision Detergents Pak I) to set up cryo-EM grids ...
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No products found
because this supplier's products are not listed.
Kacey Mersch, et al.,
bioRxiv - Biophysics 2020
Quote:
... The glass was cleaned by filling the slide holders with 0.1% Micro-90 detergent (Cole-Parmer) and sonicating for 30 minutes before rinsing the slides fifteen times with de-ionized water ...
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No products found
because this supplier's products are not listed.
Daniele Procissi, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Sections were blocked with 5% goat serum in tris-buffered saline and 0.1% Tween® 20 detergent (TBST) for 30 min and stained with anti-HER2 antibodies at 1:500 dilution (Cell Signaling #21650). After three washes with TBST ...
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No products found
because this supplier's products are not listed.
Adam G. Kreutzer, et al.,
bioRxiv - Biochemistry 2020
Quote:
A proprietary buffer containing detergent from BPS Bioscience was used for the inhibition assays ...
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No products found
because this supplier's products are not listed.
Y. Zhou, et al.,
bioRxiv - Neuroscience 2020
Quote:
... all probes were cleaned by soaking in a solution of 7% detergent (Contrad 70 Liquid Detergent; Decon Labs; King of Prussia, PA) in deionized water followed by rinsing with deionized water.
-
No products found
because this supplier's products are not listed.
Miguel Ramirez, et al.,
bioRxiv - Neuroscience 2021
Quote:
... cells were lysed in mild non-ionic detergents (0.1% Triton X-100 and Deoxycholate) and protease inhibitor cocktail (Calbiochem) to preserve the integrity of histones harbouring epitopes of interest during cell lysis ...
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No products found
because this supplier's products are not listed.
Madison A. DeWinter, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... After surfactant removal with the acidic protocol of the ProteoSpin detergent removal and sample concentration kit (Norgen Biotek 22800), samples were injected into a Bruker Elute UPLC equipped with an ACQUITY UPLC Peptide BEH C18 Column ...
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No products found
because this supplier's products are not listed.
Alex G. Johnson, et al.,
bioRxiv - Microbiology 2023
Quote:
... and pellets were resuspended using 1/5 or 1/10 dilutions of detergents stocks from the JBScreen (Jena Bioscience) or Detergent Screen (Hampton) ...
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No products found
because this supplier's products are not listed.
Felix Klingelhuber, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The supernatant was then diluted using 3x the volume of Co-IP buffer without detergent and incubated with washed GFP-Trap Magnetic Agarose (Chromotek) for 1h at 4°C on an end-over-end rotator ...
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No products found
because this supplier's products are not listed.
Natsumi Takei, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Then the extracts were diluted with Detergent Solution (MBL International) and centrifuged at 3,000 g for 3 min at 4°C ...
-
No products found
because this supplier's products are not listed.
Yue Lu, et al.,
bioRxiv - Cell Biology 2024
Quote:
Detergent-extracted cells were fixed with 2% glutaraldehyde (Ted Pella; 18426) prepared in PEM buffer at room temperature for 30 min ...
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No products found
because this supplier's products are not listed.
Ana Carreras Mascaro, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Detergents were removed / precipitated in 0.5 % trifluoroacetic acid (Riedel-de Haën, Honeywell Research Chemicals) and the protein digests were washed twice with water saturated ethylacetate (Merck) ...
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No products found
because this supplier's products are not listed.
Juan Jesus Vicente, et al.,
bioRxiv - Cell Biology 2024
Quote:
... then washed 2x with same buffer except NP-40 replaced by 1% PPS detergent (Expedeon). Immunoprecipitated complexes were released from beads with 0.2 M acetic acid and dried.
-
No products found
because this supplier's products are not listed.
Henry Ma, et al.,
bioRxiv - Immunology 2021
Quote:
... Liquid and surface disinfection was performed using Vesphene IIse detergent (dilution, 1:128; Steris Corporation, Erie, PA), while solid wastes ...
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No products found
because this supplier's products are not listed.
Manindra Bera, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The samples were further dialyzed overnight in 5X buffer without detergent using a regenerated cellulose membrane (Spectrum Labs) on a flow dialysis with a 6-8 kDa molecular weight cut off.
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No products found
because this supplier's products are not listed.
Xiaomeng Han, et al.,
bioRxiv - Neuroscience 2023
Quote:
... double immunofluorescence labeling with detergent was performed with the anti-Aβ mAb 4G8 conjugated with Alexa Fluor 647 (BioLegend) and R3VQ conjugated with 5-TAMRA (see Sup ...
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No products found
because this supplier's products are not listed.
Masahiro Enomoto, et al.,
bioRxiv - Pathology 2024
Quote:
Detergent-extracted brain homogenates [10% (w/v)] were prepared in PBS using a gentle-MACS Octo Dissociator (Miltenyi BioTec). Nine volumes of brain homogenate were then mixed with one volume of 10× detergent buffer [5% (v/v ...
-
No products found
because this supplier's products are not listed.
Kimberley A. Lewis, et al.,
bioRxiv - Microbiology 2022
Quote:
... Filters were then transferred into a 1.5 ml tube and the filter-associated cells were resuspended by adding 1 ml 1X PBS + 0.05% Triton X-100 detergent and agitating the tubes at high speed for 2 minutes using a Genie Disruptor (Zymo). This suspension as well as the inoculum were diluted ...
-
No products found
because this supplier's products are not listed.
Sebastian A. Pace, et al.,
bioRxiv - Neuroscience 2020
Quote:
... the tissue was incubated overnight in mouse polyclonal anti-CaMKIIα (1:200 in detergent-free blocking solution, GeneTex, Irvine, CA). The next day ...
-
No products found
because this supplier's products are not listed.
Jie Li, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Nuclear extracts with salt adjustment (150 mM KCl) and detergent supplement (0.1% IGEPAL CA-630) were incubated with primary antibodies (CREBBP: Santa Cruz SC-369 ...
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No products found
because this supplier's products are not listed.
Jon V. Busto, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Unbound material was removed by washing the samples 5x for 5min with head over head rotation at 4°C in wash buffer plus detergent (1x buffer XT, 1% digitonin, 2 mM PMSF and 10% glycerol) using a magnetic separator (New England Biolabs). Bound material was finally eluted with Biotin elution buffer (1x buffer Biotin XT elution ...
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No products found
because this supplier's products are not listed.
Mallory L. Myers, et al.,
bioRxiv - Microbiology 2024
Quote:
In vitro assembled spiked nanobicelles (IA-SNB) were assembled from detergent disrupted influenza virus:A/Puerto Rico/8/34 (H1N1) (Charles River) that was subsequently treated with detergent removing Bio-Beads SM-2 resin (Bio-Rad) ...
-
No products found
because this supplier's products are not listed.
Nirupa Nagaratnam, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The supernatant containing detergent solubilized His-tagged FopA was filtered through a 0.22 μm cellulose acetate membrane filter (Foxx Life Sciences) and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP ...
-
No products found
because this supplier's products are not listed.
Michael P. Dalton, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Protein was then mixed 1:1 to a final protein concentration of 2.5 nM in detergent buffer containing serially diluted 3H-labeled DTBZ (American Radiolabeled Chemicals) starting at 60 nM final concentration ...
-
No products found
because this supplier's products are not listed.
Dmitri R. Davydov, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The detergent present in the incubation mixture with BPS was removed using a DetergentOUT™ spin column (G-Biosciences, St Louis, MO). The protein was concentrated to 25-30 µM with the use of a Centrisart I MWCO 100 kDa concentrator (Sartorius AG ...
-
No products found
because this supplier's products are not listed.
Mai M. Abdelmoaty, et al.,
bioRxiv - Immunology 2021
Quote:
... followed by 1 hour incubation in 3% nonfat milk in Tris-buffered saline with 0.1% Tween 20 detergent (TBST) buffer with horseradish peroxidase-conjugated anti-rabbit (1:2000, R&D Systems, HAF008) or mouse (1:2000 ...
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No products found
because this supplier's products are not listed.
Chengcheng Fan, et al.,
bioRxiv - Immunology 2022
Quote:
... and 3 µL of the complex/detergent mixture was immediately applied to QuantiFoil 300 mesh 1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA) that had been freshly glow discharged with PELCO easiGLOW (Ted Pella ...
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No products found
because this supplier's products are not listed.
Laura A. Hehl, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The detergent was added shortly before applying 3 μL of UBR5Dimer∼UbD∼UBE2D2 to freshly glow-discharged holey carbon grids (Quantifoil, R1.2/1.3, 200 mesh). The sample was consequently plunge-frozen into liquid ethane at 95 % humidity and 4°C (blot force 3 ...