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No products found
because this supplier's products are not listed.
Hiroki Hayashi, et al.,
bioRxiv - Immunology 2020
Quote:
... and then human ACE2 recombinant protein (0.25 μg/ml, His tag; Sino Biological) was added to the wells and incubated for 2 h at RT ...
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No products found
because this supplier's products are not listed.
Stephanie Frohn, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Changrui Lu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 100 µL biotinylated recombinant human ACE2 protein (His AVI Tag, 0.25 µg/µL, 1:100) (Sinobiology) and recombinant streptavidin protein (phycoerythrin) (Abcam) were used as the primary and secondary antibodies for ACE-2 protein detection ...
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No products found
because this supplier's products are not listed.
Jacob R. Hambrook, Patrick C. Hanington,
bioRxiv - Immunology 2022
Quote:
... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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No products found
because this supplier's products are not listed.
Stephanie Hufnagel, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... MA) and recombinant mouse PD-1 His-tag protein was from R&D Systems (Minneapolis, MN). Glycine ...
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No products found
because this supplier's products are not listed.
Jinchao Chen, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Recombinant proteins were purified using the His-tag Protein Purification Kit (Ni-NTA Agarose, QIAGEN GmbH), GST-tag Protein Purification Kit (Glutathione Sepharose ...
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No products found
because this supplier's products are not listed.
Simona Selberg, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
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No products found
because this supplier's products are not listed.
Adel Avetisyan, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... His-tagged proteins in soluble fraction were purified using cOmplete His-Tag Purification Columns (Roche). The columns were washed with 10 column volumes of wash buffer 1 (20 mM Tris ...
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No products found
because this supplier's products are not listed.
Laurie Arnaud, et al.,
bioRxiv - Neuroscience 2021
Quote:
... as human recombinant proteins (Peprotech). APOE3 was resuspended in 20 mM Sodium Phosphate ...
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No products found
because this supplier's products are not listed.
Beata M. Walter, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The CobB protein was separated from His tag on His-Trap column (GE Healthcare), concentrated at 4 °C to 6 mg ml-1 ...
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No products found
because this supplier's products are not listed.
Kaito Nagashima, et al.,
bioRxiv - Immunology 2021
Quote:
... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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No products found
because this supplier's products are not listed.
Binhan Hao, Wenjie Zhou, Steven M. Theg,
bioRxiv - Biochemistry 2022
Quote:
... anti-His tag (Genscript) or anti-FLAG (Genscript ...
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No products found
because this supplier's products are not listed.
Seok-Hoon Lee, et al.,
bioRxiv - Genetics 2024
Quote:
... pRG2 (Addgene no. 104174) plasmid was digested with BsaI (New England Biolabs ...
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No products found
because this supplier's products are not listed.
Tomoyuki Nishikawa, et al.,
bioRxiv - Immunology 2021
Quote:
... 96-well plates were coated with human ACE2 recombinant protein (1μg/ml, mFc tag; #83986, Cell Signaling Technology), and then blocked using PBS containing 5% skim milk for 2 h at room temperature ...
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No products found
because this supplier's products are not listed.
Anna Balcerak, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Purified HAX1 protein (Proteintech, Ag27244, fused with His-tag) was labeled with RED-NHS 2nd Generation dye according to the supplied labeling protocol Monolith NT™ Protein Labeling Kit ...
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No products found
because this supplier's products are not listed.
Takumi Takeda, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Purification of His-tagged proteins was carried out using His-tag affinity resin (His-resin) (Clontech, CA, USA).
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No products found
because this supplier's products are not listed.
Stefania Capone, et al.,
bioRxiv - Immunology 2020
Quote:
... recombinant human His-tagged ACE-2 protein (RayBiotech) was incubated on cells before adding any antibodies ...
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No products found
because this supplier's products are not listed.
Kazuhiro Yamamoto, et al.,
bioRxiv - Cell Biology 2022
Quote:
... recombinant human HMGB2 (C-terminal His tag, TP720732) from ORIGENE (Rockville, MD); anti-rabbit alkaline phosphatase-linked antibody ...
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No products found
because this supplier's products are not listed.
Chaelin Lee-Eom, et al.,
bioRxiv - Biophysics 2024
Quote:
Full-length human tau protein with a 6x His-tag was cloned into the pRK172 vector and expressed in LEMO21 cells (NEB #C2528J). Cells were cultured in TB media at 37 °C until the OD600 reached 0.6 ...
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No products found
because this supplier's products are not listed.
Sandro Trova, et al.,
bioRxiv - Microbiology 2023
Quote:
... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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No products found
because this supplier's products are not listed.
Farah Haque, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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No products found
because this supplier's products are not listed.
Deepti Mudaliar, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Recombinant Human CKII alpha prime polypeptide His Protein (NBP2-22736) was purchased from Novus Biologicals (Centennial, CO, USA). CK2 Substrate (synthetic peptide RRRADDSDDDDD ...
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No products found
because this supplier's products are not listed.
Tatiana P. Soares da Costa, et al.,
bioRxiv - Microbiology 2022
Quote:
... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
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No products found
because this supplier's products are not listed.
Katarzyna Olga Rojek, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Human recombinant VEGF-165 (Stemcell technologies; Saint Egrève ...
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No products found
because this supplier's products are not listed.
Simone Giaveri, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... His-tag proteins have been purified by using MagneHis system (Promega) and DynaMag spin magnet (Thermo Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Steven Swendeman, et al.,
bioRxiv - Biochemistry 2022
Quote:
... and His-tag (Santa Cruz) expression (data not shown) ...
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No products found
because this supplier's products are not listed.
Changlong Chen, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Protein purity was verified by protein gel electrophoresis followed by coomassie staining and western blotting using anti-His (HRP) antibody (Miltenyi Biotec). Signals were generated by chemiluminescence using Clarity ECL Western Blotting Substrate (BioRad ...
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No products found
because this supplier's products are not listed.
Mireia Perez Verdaguer, et al.,
bioRxiv - Systems Biology 2022
Quote:
Recombinant human EGF was purchased from BD Biosciences (San Jose ...
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No products found
because this supplier's products are not listed.
Shiho Tanaka, et al.,
bioRxiv - Immunology 2021
Quote:
... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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No products found
because this supplier's products are not listed.
Nur F. Isa, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Lie Wang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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No products found
because this supplier's products are not listed.
Jinkai Zang, et al.,
bioRxiv - Microbiology 2020
Quote:
... recombinant RBD protein containing a C-terminal 6xHis tag was formulated with the Alhydrogel adjuvant (Invivogen) and each vaccine dose contained 5 μg of RBD and 500 μg of aluminum hydroxide ...
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No products found
because this supplier's products are not listed.
Ines A Cadena, et al.,
bioRxiv - Bioengineering 2024
Quote:
... and recombinant human insulin 0.5%) (Lonza). Human cervical cancer cell line SiHa from ATCC (ATCC® HTB-35™ ...
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No products found
because this supplier's products are not listed.
Michael Udoh, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... the purity of each sample was verified by analytical chiral column HPLC (Lux® Amylose, Phenomenex, Supplementary Figure 1), and their structures were also verified by NMR and mass spectrometry ...
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No products found
because this supplier's products are not listed.
Cameron Geller, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... human recombinant EGF (Corning, Bedford, MA), hydrocortisone (BD Biosciences ...
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No products found
because this supplier's products are not listed.
Carolyn Rydyznski Moderbacher, et al.,
bioRxiv - Immunology 2022
Quote:
... the hACE2 receptor bound to the S protein was then detected using a mouse anti-His-Tag horseradish peroxidase conjugate (Southern Biotech) and a colorimetric signal generated by the addition of 3,3′,5,5′-TMB substrate ...
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No products found
because this supplier's products are not listed.
M. T. Gyparaki, et al.,
bioRxiv - Cell Biology 2020
Quote:
... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
-
No products found
because this supplier's products are not listed.
Shraddha Pai, et al.,
bioRxiv - Genomics 2021
Quote:
We downloaded publicly-available Hi-C data from human prefrontal cortex tissue23,24 (Illumina HiSeq 2000 paired-end raw sequence reads ...
-
No products found
because this supplier's products are not listed.
Hisashi Nomura, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were incubated with 10 μg of rDsg3-E-His in 100 μL of RPMI on ice for 1 h and stained with anti-E-tag-FITC (Bethyl Laboratories). After excluding dead cells by staining with 7AAD ...
-
No products found
because this supplier's products are not listed.
Sarah A. Shelby, Thomas R. Shaw, Sarah L. Veatch,
bioRxiv - Biophysics 2022
Quote:
... VCAM-1/Fc chimeric proteins and anti-human Fc antibodies (recombinant human VCAM-1/Fc chimera protein, Fisher 862-VC-100; Fcγ-specific goat anti-human IgG, Jackson Immunoresearch 109-005-008) or poly-L-lysine (Sigma P4707).
-
No products found
because this supplier's products are not listed.
Valentina Marchesin, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... The measurements were performed on a HPLC Nexera X2 HPLC (Shimadzu) coupled to an API 5500TM (AB Sciex) with positive ion electrospray ionization and operated in multiple reaction monitoring (MRM ...
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No products found
because this supplier's products are not listed.
Alexander W. Justin, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Green fluorescent protein (GFP) and Red Fluorescent Protein (RFP) human umbilical vein endothelial cells (HUVECs, Promocell), normal human lung fibroblasts (NHLFs ...
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No products found
because this supplier's products are not listed.
Xiaoning Yang, et al.,
bioRxiv - Immunology 2023
Quote:
Recombinant human PD-1 protein was biotinylated and loaded using SA sensor (Pall Corporation). Six concentrations of Finotonlimab and Nivolumab were added for real-time association and dissociation analysis using Octet system ...
-
No products found
because this supplier's products are not listed.
A.A. Shaldzhyan, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
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No products found
because this supplier's products are not listed.
Teddy Kamata, Chun-Song Yang, Bryce M. Paschal,
bioRxiv - Biochemistry 2021
Quote:
... recombinant GST-tagged tandem AF1521 was fluorescently labeled using IRDye® 800CW Protein Labeling Kit (LI-COR Biosciences, Lincoln, NE, USA). Prepared samples were run on SDS-PAGE ...
-
No products found
because this supplier's products are not listed.
Therese de Neergaard, et al.,
bioRxiv - Immunology 2022
Quote:
Human monocytic cell line Tamm–Horsfall protein 1 (THP-1) (TIB-202, male; American Type Culture Collection) was cultured in RPMI 1640 medium (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Vivian K Lee, et al.,
bioRxiv - Bioengineering 2024
Quote:
... Human astrocytes (Sciencell) and human brain vascular pericytes (Sciencell ...
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No products found
because this supplier's products are not listed.
Camilla Kjeldgaard Larsen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).
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No products found
because this supplier's products are not listed.
Sanjib Saha, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Protein expression was normalized to total protein (DM-TP01, Protein Simple) and compared between tissue and cell types.
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No products found
because this supplier's products are not listed.
Michał A. Surma, et al.,
bioRxiv - Biochemistry 2021
Quote:
... healthy diets differing by protein content: Teklad global 14% protein (Envigo #2014S, low protein) and Teklad global 18% protein (Envigo #2018S ...