-
No products found
because this supplier's products are not listed.
Juwel Chandra Baray, et al.,
bioRxiv - Immunology 2020
Quote:
... S2 ECD-His Recombinant Protein (Sino Biological, China), and RBD-His Recombinant Protein (Sino Biological ...
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No products found
because this supplier's products are not listed.
Ambra Cappelletto, et al.,
bioRxiv - Pathology 2021
Quote:
... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
-
No products found
because this supplier's products are not listed.
Jacob R. Hambrook, Patrick C. Hanington,
bioRxiv - Immunology 2022
Quote:
... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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No products found
because this supplier's products are not listed.
Poshan V. Pokharel, et al.,
bioRxiv - Neuroscience 2024
Quote:
... primary antibody for p75NTR-ECD (Cell Signaling Technology, Cat No. 8238), secondary antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Changrui Lu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 100 µL biotinylated recombinant human ACE2 protein (His AVI Tag, 0.25 µg/µL, 1:100) (Sinobiology) and recombinant streptavidin protein (phycoerythrin) (Abcam) were used as the primary and secondary antibodies for ACE-2 protein detection ...
-
No products found
because this supplier's products are not listed.
Thomas Mandel Clausen, et al.,
bioRxiv - Microbiology 2020
Quote:
... and SARS-CoV-2 spike protein (ECD, His & Flag Tag) (GenScript Z03481). Proteins were biotinylated using EZ-Link™ Sulfo-NHS-Biotin ...
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No products found
because this supplier's products are not listed.
Jinchao Chen, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Recombinant proteins were purified using the His-tag Protein Purification Kit (Ni-NTA Agarose, QIAGEN GmbH), GST-tag Protein Purification Kit (Glutathione Sepharose ...
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No products found
because this supplier's products are not listed.
Adel Avetisyan, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... His-tagged proteins in soluble fraction were purified using cOmplete His-Tag Purification Columns (Roche). The columns were washed with 10 column volumes of wash buffer 1 (20 mM Tris ...
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No products found
because this supplier's products are not listed.
Simona Selberg, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
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No products found
because this supplier's products are not listed.
Kelsey R. Nickerson, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... recombinant mNope-ECD-Fc protein (Biotechne/R&D Systems, 1294-NP) was added at 25 μg/ml (≍ 0.2 μM ...
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No products found
because this supplier's products are not listed.
Beata M. Walter, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The CobB protein was separated from His tag on His-Trap column (GE Healthcare), concentrated at 4 °C to 6 mg ml-1 ...
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No products found
because this supplier's products are not listed.
Anna Balcerak, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Purified HAX1 protein (Proteintech, Ag27244, fused with His-tag) was labeled with RED-NHS 2nd Generation dye according to the supplied labeling protocol Monolith NT™ Protein Labeling Kit ...
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No products found
because this supplier's products are not listed.
Xin Wang, et al.,
bioRxiv - Immunology 2022
Quote:
... NGFR (345109, Biolegend), ITGA6 (313628 ...
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No products found
because this supplier's products are not listed.
Takumi Takeda, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Purification of His-tagged proteins was carried out using His-tag affinity resin (His-resin) (Clontech, CA, USA).
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No products found
because this supplier's products are not listed.
Veronica H. Ryan, et al.,
bioRxiv - Biochemistry 2020
Quote:
... insoluble His-tag purification (Addgene ID ...
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No products found
because this supplier's products are not listed.
B. Pardo-Rodríguez, et al.,
bioRxiv - Cell Biology 2024
Quote:
... and NGFR (p75NTR) 1:250 (#Sc-271708, Santa Cruz Biotechnology) were used ...
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No products found
because this supplier's products are not listed.
Xinhao Hou, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The recombinant proteins were affinity purified through GST tag binding to amylose resin (BioLabs) according to the manufacturer’s instructions.
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No products found
because this supplier's products are not listed.
Sandro Trova, et al.,
bioRxiv - Microbiology 2023
Quote:
... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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No products found
because this supplier's products are not listed.
Simone Giaveri, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... His-tag proteins have been purified by using MagneHis system (Promega) and DynaMag spin magnet (Thermo Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Kazuhiro Yamamoto, et al.,
bioRxiv - Cell Biology 2022
Quote:
... recombinant human HMGB2 (C-terminal His tag, TP720732) from ORIGENE (Rockville, MD); anti-rabbit alkaline phosphatase-linked antibody ...
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No products found
because this supplier's products are not listed.
Azali Azlan, Li Zhu, Ryuya Fukunaga,
bioRxiv - Genetics 2024
Quote:
... The rabbit polyclonal anti-Sakura antibodies were affinity purified using His-MBP-Sakura recombinant protein and Affigel-l5 (Bio-rad), following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
A-M C Overstreet, et al.,
bioRxiv - Microbiology 2023
Quote:
... coli were made from an overnight agar culture and incubated with 3 mM HMGB1 recombinant protein containing a HIS tag (Abclonal) or an hFc tag (Sino Biological ...
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No products found
because this supplier's products are not listed.
Farah Haque, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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No products found
because this supplier's products are not listed.
Laurie Arnaud, et al.,
bioRxiv - Neuroscience 2021
Quote:
... as human recombinant proteins (Peprotech). APOE3 was resuspended in 20 mM Sodium Phosphate ...
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No products found
because this supplier's products are not listed.
Shiho Tanaka, et al.,
bioRxiv - Immunology 2021
Quote:
... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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No products found
because this supplier's products are not listed.
Olena Parilova, Piia Bartos, Anssi M. Malinen,
bioRxiv - Biochemistry 2024
Quote:
... Fractions and final Rrn3 preparations were quality controlled by SDS-PAGE and Western analysis using Coomassie staining and monoclonal anti-His-tag antibody ( 6X His Tag Antibody Dylight™ 800 Conjugated, clone 33D10.D2, cat.no. 200-345-382, Rockland Immunochemicals), respectively.
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No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ...
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No products found
because this supplier's products are not listed.
Deepti Mudaliar, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Recombinant Human CKII alpha prime polypeptide His Protein (NBP2-22736) was purchased from Novus Biologicals (Centennial, CO, USA). CK2 Substrate (synthetic peptide RRRADDSDDDDD ...
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No products found
because this supplier's products are not listed.
Eun Hye Kim, et al.,
bioRxiv - Microbiology 2022
Quote:
... NGFR was detected with a 20 μl per million cells of Alexa Flour 647 conjugated α-NGFR (BD Bioscience), and V5 detected with a 1:100 dilution of fluorescein isothiocyanate (FITC)-conjugated α-mV5 (Invitrogen ...
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No products found
because this supplier's products are not listed.
Lie Wang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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No products found
because this supplier's products are not listed.
Davide Monteferrario, et al.,
bioRxiv - Bioengineering 2024
Quote:
... CD271/NGFR (Miltenyi Biotec) MHC-I and MHC-II (BD Biosciences ...
-
No products found
because this supplier's products are not listed.
Carole Seguin-Devaux, et al.,
bioRxiv - Immunology 2024
Quote:
... or rabbit anti-His (Bethyl, ImTec Diagnostic NV ...
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No products found
because this supplier's products are not listed.
Krystal C. Johnson, et al.,
bioRxiv - Biochemistry 2023
Quote:
... recombinant AGO2 protein (Active Motif) was purchased and serially diluted in Protein LoBind tubes (Eppendorf ...
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No products found
because this supplier's products are not listed.
Nur F. Isa, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
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No products found
because this supplier's products are not listed.
M. T. Gyparaki, et al.,
bioRxiv - Cell Biology 2020
Quote:
... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
-
No products found
because this supplier's products are not listed.
Ferdinand Meneau, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... Recombinant proteins and OA (Enzo Life Sciences) were diluted in PBS and centrifuged at 14,000 rpm at 4°C for 5 minutes before injection ...
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No products found
because this supplier's products are not listed.
Carolyn Rydyznski Moderbacher, et al.,
bioRxiv - Immunology 2022
Quote:
... the hACE2 receptor bound to the S protein was then detected using a mouse anti-His-Tag horseradish peroxidase conjugate (Southern Biotech) and a colorimetric signal generated by the addition of 3,3′,5,5′-TMB substrate ...
-
No products found
because this supplier's products are not listed.
Fangzhu Zhao, et al.,
bioRxiv - Bioengineering 2021
Quote:
6x-His tag antibodies were coated at 2 ug/mL in PBS onto 96-well half-area high binding plates (Corning, 3690) overnight at 4°C or 2 h at 37°C ...
-
No products found
because this supplier's products are not listed.
J. Ignacio Gutiérrez, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... which was then blocked with 5% nonfat milk and incubated with a rabbit IgG primary antibody (which binds to the protein A moiety of the TAP tag) for 1 hour and then with fluorescently labelled goat anti-rabbit secondary antibody IRdye 680RD goat-anti-rabbit (LI-COR Biosciences Cat# 926–68071 ...
-
No products found
because this supplier's products are not listed.
Roberto Espinoza-Corral, et al.,
bioRxiv - Plant Biology 2023
Quote:
... 150 mM NaCl and 0.01% tween-20) overnight at 4°C (anti-PsbA; AS05 084A; anti-PsaB; AS10 695; anti-APC; AS08 277; Agrisera, anti-His tag; TA150087; OriGene). The membrane was washed 3 times in TBST-T at room temperature for 15 minutes each wash followed by incubation with secondary polyclonal anti-rabbit antisera HRP for one hour at room temperature in TBS-T (Jackson ImmunoResearch ...
-
No products found
because this supplier's products are not listed.
Sonali Jindal, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... anti-rabbit (Protein Simple # 042-206, RTU) or anti-mouse (Protein Simple# 042-205 ...
-
No products found
because this supplier's products are not listed.
Ameen A. Salahudeen, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... retroorbitally and serum was analyzed by Western blot for expression of FGFR1-ECD-Fc or Fc using anti-IgG2α Fc (Jackson ImmunoResearch). All adenoviral inserts were cloned into the E1 region of E1- E3- Ad strain 5 by homologous recombination and amplified in in 293 cells followed by CsCl2 gradient purification of virus ...
-
No products found
because this supplier's products are not listed.
Brandon Podyma, et al.,
bioRxiv - Physiology 2020
Quote:
... Ngfr-KO mice were purchased from Jackson Labs (Bar Harbor, ME, USA) (#002213)(19) ...
-
No products found
because this supplier's products are not listed.
S. M. Nayeemul Bari, et al.,
bioRxiv - Microbiology 2021
Quote:
... and tag-free proteins were collected and concentrated using a 10K MWCO centrifugal filter (PALL). Concentrated proteins were further purified by size exclusion chromatography using Superdex 75 Increase 10/300 GL (Cytiva ...
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No products found
because this supplier's products are not listed.
Silvio Schmidt, et al.,
bioRxiv - Neuroscience 2020
Quote:
... the S100 calcium-binding protein B (S100B; rabbit, Synaptic system), and BrdU (mice ...
-
No products found
because this supplier's products are not listed.
A.A. Shaldzhyan, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
-
No products found
because this supplier's products are not listed.
Camilla Kjeldgaard Larsen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).
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No products found
because this supplier's products are not listed.
Xinyi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... but with different rabbit polyclonal antibodies from the Human Protein Atlas (HPA) purchased from Atlas Antibodies. On the next day ...
-
No products found
because this supplier's products are not listed.
Michał A. Surma, et al.,
bioRxiv - Biochemistry 2021
Quote:
... healthy diets differing by protein content: Teklad global 14% protein (Envigo #2014S, low protein) and Teklad global 18% protein (Envigo #2018S ...