-
No products found
because this supplier's products are not listed.
Ann Logan, et al.,
bioRxiv - Immunology 2022
Quote:
... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
-
No products found
because this supplier's products are not listed.
Ferdinando M. Milazzo, et al.,
bioRxiv - Microbiology 2022
Quote:
... 1.0 μg/mL human ACE2 protein mouse Fc-tag (Sino Biological) was added and incubated for 1 h at 37 °C ...
-
No products found
because this supplier's products are not listed.
Julius Benicky, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... with C-terminal 6xHis tag produced in HEK293 cells and human recombinant PD-L1-Fc chimera protein produced in mouse NS0 cells were obtained from R&D Systems, Minneapolis ...
-
No products found
because this supplier's products are not listed.
Duyen Huynh, et al.,
bioRxiv - Biophysics 2024
Quote:
... and the virus envelope plasmid pMD2.G (Addgene #12259) using JetPRIME (Polyplus-transfection ...
-
No products found
because this supplier's products are not listed.
Minyue Qiu, et al.,
bioRxiv - Microbiology 2022
Quote:
... sections were incubated with anti-dengue virus 1+2+3+4 primary antibodies (ab26837, Abcam, England) at 4°C overnight ...
-
No products found
because this supplier's products are not listed.
Juliet O. Obi, et al.,
bioRxiv - Biophysics 2024
Quote:
The first 70 nucleotides corresponding to SLA from the dengue virus serotype 2 genome (New Guinea C strain, GenBank no AF038403.1) was synthesized by GenScript in non-biotin and biotin-tagged forms ...
-
No products found
because this supplier's products are not listed.
Maria Vittoria Mancini, et al.,
bioRxiv - Microbiology 2020
Quote:
... Primary antibody for DENV was MAB8705 Anti-dengue virus complex antibody (Millipore); secondary antibody was the Goat anti-mouse Alexa Fluor 488 ...
-
No products found
because this supplier's products are not listed.
Emily Speranza, et al.,
bioRxiv - Immunology 2021
Quote:
... Human Fc Block (BD) was added to all surface staining cocktails ...
-
No products found
because this supplier's products are not listed.
Michele Martins, et al.,
bioRxiv - Biochemistry 2020
Quote:
... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).
-
No products found
because this supplier's products are not listed.
Yanting Duan, et al.,
bioRxiv - Immunology 2022
Quote:
... and the DAXL-88 (anti-AXL) binding assay used Alexa Fluor 647 AffiniPure anti-Fc tag Goat Anti-Human IgG (410711, BioLegend). Anti-Human CD4 antibody (BD ...
-
No products found
because this supplier's products are not listed.
Wei Zhang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Adeno-associated virus (AAV) cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...
-
No products found
because this supplier's products are not listed.
Déborah Lécuyer, et al.,
bioRxiv - Microbiology 2023
Quote:
... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
-
No products found
because this supplier's products are not listed.
Ayijiang Yisimayi, et al.,
bioRxiv - Immunology 2023
Quote:
Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
-
No products found
because this supplier's products are not listed.
Sarah A. Shelby, Thomas R. Shaw, Sarah L. Veatch,
bioRxiv - Biophysics 2022
Quote:
... VCAM-1/Fc chimeric proteins and anti-human Fc antibodies (recombinant human VCAM-1/Fc chimera protein, Fisher 862-VC-100; Fcγ-specific goat anti-human IgG, Jackson Immunoresearch 109-005-008) or poly-L-lysine (Sigma P4707).
-
No products found
because this supplier's products are not listed.
Jasmine Moshiri, et al.,
bioRxiv - Microbiology 2020
Quote:
... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
-
No products found
because this supplier's products are not listed.
Fang Tian, et al.,
bioRxiv - Biophysics 2021
Quote:
... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... human IL-12p70 (HEK293) (Peprotech) and 50ng/ml recombinant human IL-18 (MBL International ...
-
No products found
because this supplier's products are not listed.
René Platzer, et al.,
bioRxiv - Immunology 2023
Quote:
... coli serotype O55:B5 (Merck) for 24 to 48 hours to arrive at a mature BMDC culture or alternatively frozen to -80°C in 90% FCS (Biowest ...
-
No products found
because this supplier's products are not listed.
George N. Llewellyn, et al.,
bioRxiv - Microbiology 2023
Quote:
... mouse-anti-human IgG Fc-HRP (Southern Biotech) was diluted 1:2000 in blocking buffer and added and plates were incubated for 2 hours at 370C ...
-
No products found
because this supplier's products are not listed.
Toufic Kassouf, et al.,
bioRxiv - Cell Biology 2023
Quote:
... with virus packaging and envelope expressing plasmids using Fugene-6 (Roche). Medium was replaced with DMEM with 10% FCS the next day and supernatant was harvested 3 days post-transfection ...
-
No products found
because this supplier's products are not listed.
Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
HEK293 (human, DMSZ, ACC 635) and U2OS (human, American Type Culture Collection [ATCC], HTB-96) cell were cultivated in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Qiwang Xiang, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... SARS-CoV-2 Envelope protein (Cell Signaling Technology, 74698S); furin (Proteintech ...
-
No products found
because this supplier's products are not listed.
Rinshi S. Kasai, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
-
No products found
because this supplier's products are not listed.
Wei-Li Ling, Samuel Ken-En Gan,
bioRxiv - Immunology 2022
Quote:
KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
-
No products found
because this supplier's products are not listed.
Adrian Escobar, et al.,
bioRxiv - Cell Biology 2022
Quote:
... For protein-protein interaction experiments using the pCMV6 vector with HA or Myc-DDK peptide tag at the 3’-end (Origene Technologies), we used the same IF cloning system to subclone cDNAs of our genes-of-interests (GOIs ...
-
No products found
because this supplier's products are not listed.
Jérôme Wahis, et al.,
bioRxiv - Neuroscience 2020
Quote:
... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
-
No products found
because this supplier's products are not listed.
Coralie F. Daussy, et al.,
bioRxiv - Microbiology 2020
Quote:
... human Fc blocking reagent (MACS Miltenyi Biotec) was added to this blocking step ...
-
No products found
because this supplier's products are not listed.
Viola Maria Francesca, et al.,
bioRxiv - Immunology 2022
Quote:
... supplemented with fetal calf serum (FCS, 3%, Lonza) and HEPES (1.8%) ...
-
No products found
because this supplier's products are not listed.
Yadira M. Soto-Feliciano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human HEK293 cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) ...
-
No products found
because this supplier's products are not listed.
María José Lista, et al.,
bioRxiv - Microbiology 2021
Quote:
... Proteins were visualized by LI-COR (Odyssey Fc) measuring secondary antibody fluorescence or using Amersham ECL Prime Western Blotting Detection reagent (GE Lifesciences ...
-
No products found
because this supplier's products are not listed.
Sofya A. Polyanskaya, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ...
-
No products found
because this supplier's products are not listed.
Maud Dupont, et al.,
bioRxiv - Microbiology 2023
Quote:
... Flag-tag (Proteintech, 66008-3-ig), HA-tag (Proteintech ...
-
No products found
because this supplier's products are not listed.
Peng He, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... cDNA libraries were then tagmented using the Illumina/Nextera DNA prep kit (FC 121-1030) with index tags from Illumina (FC 121-1031), cleaned up with Ampure XP beads ...
-
No products found
because this supplier's products are not listed.
Kyung-Tae Lee, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
-
No products found
because this supplier's products are not listed.
Sabine Brumm, et al.,
bioRxiv - Plant Biology 2024
Quote:
... m-IgG Fc binding protein HRP (sc-542732, Santa Cruz biotechnology) 1:5000 and goat anti-rabbit IgG HRP (AB205718 ...
-
No products found
because this supplier's products are not listed.
Veronica Locher, et al.,
bioRxiv - Immunology 2022
Quote:
Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Luca Simula, et al.,
bioRxiv - Immunology 2021
Quote:
... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with phospho-DRP1 (Ser 616 ...
-
No products found
because this supplier's products are not listed.
Christina Vrettou, et al.,
bioRxiv - Immunology 2022
Quote:
... and blotted with anti-human IgG Fc (Jackson labs). (E ...
-
No products found
because this supplier's products are not listed.
Paul-Lennard Mendez, et al.,
bioRxiv - Genomics 2024
Quote:
... starved for 3 h in EBM2 without FCS (PromoCell) and stimulated with 0.3 nM BMP9 for 90 min ...
-
No products found
because this supplier's products are not listed.
Aurélien Fouillen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... Cells were transfected 24 h later with the pRK5 plasmid coding for the human V2R (wild-type or mutant) fused at its N-terminus to the SNAP-tag (pRK5-SNAP vector, PerkinElmer Revvity). Transfections were performed with X-tremeGENE 360 (Merck) ...
-
No products found
because this supplier's products are not listed.
Brenda Kapingidza, et al.,
bioRxiv - Immunology 2023
Quote:
... AlexaFluor 700 anti-human CD38 (clone LS198-4-3, Beckman Coulter), APC-Cy7 anti-human CD19 (clone SJ25C1 ...
-
No products found
because this supplier's products are not listed.
Sonali Jindal, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... human COX2 protein (Cayman Chemical, 60122, 4ng), and 25μg cell line lysates in RIPA buffer were separated by WES automated gel electrophoresis System (Protein Simple ...
-
No products found
because this supplier's products are not listed.
Jiejun Zhu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 400nl of virus was delivered to 3 locations respectively by a microinjection system (Nanoliter 2010, WPI Ltd) through a craniotomy (<1 mm2) ...
-
No products found
because this supplier's products are not listed.
Wanlu Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... TAG (triacylglycerol, D5-TAG-Mix, LM-6000/D5-TAG 17:0,17:1,17:1, Avanti Polar Lipids), PA (phosphatidic acid ...
-
No products found
because this supplier's products are not listed.
Erik Procko,
bioRxiv - Biochemistry 2020
Quote:
Hydrated anti-human IgG Fc biosensors (Molecular Devices) were dipped in expression medium containing RBD-IgG1 for 60 s ...
-
No products found
because this supplier's products are not listed.
Jamie C. Moffa, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was removed from the upper chamber of the concentrator and stored in low protein binding tubes (Eppendorf #022431064) at -80°C.
-
No products found
because this supplier's products are not listed.
Juan Manuel García-Arcos, et al.,
bioRxiv - Biophysics 2022
Quote:
... 20 μM AACOCF3 inhibiting the nuclear envelope stretchsensitive enzyme cPLA2 (Tocris Bioscience #1462), 100 μM 2-APB blocking stretch-activated inositol triphosphate receptors (InsP3Rs ...
-
No products found
because this supplier's products are not listed.
Loyda M. Morales Rodríguez, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
HEK293 cells were plated onto 25mm coverslips (Electron Microscopy Sciences, #50949050) coated with poly-D-lysine (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
-
No products found
because this supplier's products are not listed.
Daniel W. Sorensen, et al.,
bioRxiv - Genetics 2024
Quote:
... human and fetal mouse CMs were imaged on a Cytation 3 (Biotek) or a Ti-E (Nikon) ...