-
No products found
because this supplier's products are not listed.
Jacqueline Cornean, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 0.1 % tetramethylethylenediamine (TEMED, Roth) and 0.005 % 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (4-hydroxy-TEMPO, Sigma) were added to activate the polymerization reaction ...
-
No products found
because this supplier's products are not listed.
Sam Norris, Jimmy K. Hu, Neil H Shubin,
bioRxiv - Developmental Biology 2024
Quote:
... 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPO, Thermo Scientific), and Proteinase K (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Elina Mäntylä, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and lamin A/C N-terminus residues aa 2-29 (LA/C-N, [E-1], sc-376248, Santa Cruz Biotechnology, DA, USA), a rabbit monoclonal Ab (rMAb ...
-
No products found
because this supplier's products are not listed.
Lynn Htet Htet Aung, et al.,
bioRxiv - Genetics 2020
Quote:
... Membranes were probed for 1 hour at room temperature and overnight at 4°C with primary antibody (Anti-8-Hydroxy-2’-deoxyguanosine antibody, 1:1000, Abcam) in 3% BSA ...
-
No products found
because this supplier's products are not listed.
Graham. M. Pitcher, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 3-Chloro-4-fluoro-N-[4-[[2-(phenylcarbonyl) hydrazino]carbonyl]benzyl]benzene-sulfonamide (TCN 201, 3 μM dissolved in DMSO; Tocris Bioscience), (αR,βS)-α-(4-Hydroxyphenyl)-β-methyl-4-(phenylmethyl)-1-piperidinepropanol maleate (Ro25-6981 ...
-
No products found
because this supplier's products are not listed.
Ashwin Narayanan, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... concentrations was assessed using reduction in WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]- 1,3-benzene Disulfonate) to water-soluble formazan (Roche, France). Cells were seeded in 96-well plates at 2 × 104 cells/well and treated with the different NAM concentrations at 37°C ...
-
No products found
because this supplier's products are not listed.
Lei Qiang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Next cells were incubated at 4°C overnight with anti-E-cadherin (1:200; Cell Signaling Biotechnology), β-Catenin (1:200 BD Biosciences ...
-
No products found
because this supplier's products are not listed.
Elise Marechal, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... 4-hydroxy-tamoxifen (Merck H7904) was solubilized in 100% ethanol ...
-
No products found
because this supplier's products are not listed.
Florian Noack, et al.,
bioRxiv - Genomics 2020
Quote:
... at 4°C followed by staining against Pax6 (1:40; BD Bioscience, Cat. N: 561664), Eomes (1:33 ...
-
No products found
because this supplier's products are not listed.
Dayoung Kim, James M Olson, Jonathan A Cooper,
bioRxiv - Cancer Biology 2024
Quote:
... resuspended in HBSS containing 0.5% BSA and incubated for 30 min at 4 °C with 10 μg/ml anti-N-cad primary antibody (Proteintech, 22018-1-AP). Cells were washed twice in HBSS/0.5% BSA and incubated with Alexa Fluor 647-conjugated donkey anti-rabbit IgG (H+L ...
-
No products found
because this supplier's products are not listed.
Jennifer R Klaus, et al.,
bioRxiv - Microbiology 2020
Quote:
... 4-Hydroxy-2-heptylquinoline N-oxide (HQNO) was purchased from Cayman chemicals (cat. 15159). 4-Hydroxy-3-methyl-2-nonenylquinoline (HMNQ ...
-
No products found
because this supplier's products are not listed.
Vivek K. Bajpai, et al.,
bioRxiv - Cell Biology 2021
Quote:
... mouse TYRP1 (MEL-5) (1:500; 4°C overnight; BioLegend, 917801), rabbit MITF (1:200 ...
-
No products found
because this supplier's products are not listed.
Alex J. Shamoun, et al.,
bioRxiv - Genetics 2024
Quote:
... 4) IDS-KO receiving 1 mg/kg idursulfase IV and nebulized idursulfase (KO-NEB, n = 5). The nebulized idursulfase was delivered as 167 µL of 2 mg/mL ...
-
No products found
because this supplier's products are not listed.
Remi Janicot, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
-
No products found
because this supplier's products are not listed.
Alexandra Turfe, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and five rats that were identified as significant outliers with a z-score ≥ 5 on at least one measure (n=1 F CORT Charles River, n= 2 F VEH Charles River, n=1 M VEH Taconic, n=1 M CORT Taconic). Statistical analyses were conducted on the remaining rats (Table 1).
-
No products found
because this supplier's products are not listed.
Michael J. Kimmich, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Animals were imaged for 1 min at 5 fps at 20 ± 1°C using a Ti-E microscope (Nikon) with an Andor Zyla sCMOS 5.5 camera (Oxford Instruments ...
-
No products found
because this supplier's products are not listed.
Matilde Mengkrog Holen, et al.,
bioRxiv - Genetics 2022
Quote:
Total RNA was extracted from the stomach and pyloric caeca tissue samples stored at -80 °C (n = 4 for control and fly larvae, n = 5 for shrimp shell) using the RNeasy Plus Universal Kit (QIAGEN). RNA quality was assessed using a 2100 Bioanalyzer with the RNA 6000 nano kit (Agilent ...
-
No products found
because this supplier's products are not listed.
Sarah M. Barry, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and then incubated O/N at 4°C with blocking solution and primary anti-c-Fos (226 008, Synaptic Systems, rabbit, 1:500), anti-mCherry (NBP2-25157 Novus Biologicals ...
-
No products found
because this supplier's products are not listed.
Thomas Leibing, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Table 1) was performed at 4°C overnight at a 1:1000 dilution in 5% nonfat dry milk (Bio-Rad No. 1706404). Incubation of HRP conjugated secondary antibody (different species ...
-
No products found
because this supplier's products are not listed.
John Kokkinos, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... cytokeratin (DAKO, Cat. M3515; 1:100 at 4 °C), ki67 (ThermoFisher ...
-
No products found
because this supplier's products are not listed.
Qi Qu, et al.,
bioRxiv - Physiology 2023
Quote:
... for 1 h at 4 □ on a ThermoMixer C (Eppendorf), 350 µl of water and 250 µl of chloroform were added to the mixture to induce phase separation ...
-
No products found
because this supplier's products are not listed.
Michelle Braha, et al.,
bioRxiv - Neuroscience 2020
Quote:
The study was performed in a subset of B6 mice (4 months old n=5, 10 eyes) and BALB/c mice (4 months old, n=5, 10 eyes) purchased from Jackson Labs (Bar Harbor, ME, USA). PVEPs were recorded in anesthetized mice as described in PERG methods section ...
-
No products found
because this supplier's products are not listed.
Carla Belmonte-Mateos, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... They were then incubated O/N at 4°C with the corresponding primary antibody: anti-DsRed (1:500; Clontech), mouse anti-GFP ([1:100] ...
-
No products found
because this supplier's products are not listed.
Chew Theng Lim, et al.,
bioRxiv - Biochemistry 2021
Quote:
... GST-tag and MBP-tag were attached to its N- and C-terminus respectively (Addgene ID 169195). The cleavage at G//A resulted in 25 and 42 kDa products ...
-
No products found
because this supplier's products are not listed.
Yuichiro Iwamoto, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The antibodies used for western blotting were: anti-HALO (1:1000 in 0.5% milk TBST, 4°C o/n, Promega G9211), anti-GAPDH (1:10000 in TBST ...
-
No products found
because this supplier's products are not listed.
Lissenya B. Argueta, et al.,
bioRxiv - Cell Biology 2021
Quote:
... for 1 hour at room temperature and then incubated overnight at 4°C in a humid chamber with primary antibodies (SARS-CoV-2-N, GeneTex GTX635679, at 1:100; CD163,Novus Biologicals NBP2-48846, 1:250) diluted in 1% BSA/0.1% Triton-X PBS (PBST) ...
-
No products found
because this supplier's products are not listed.
Tao Huang, et al.,
bioRxiv - Neuroscience 2021
Quote:
... incubated for 5 days at 4°C with Car4 primary antibody (1:500, RRID:AB_10013483; R&D Systems), rinsed ...
-
No products found
because this supplier's products are not listed.
Felix Wiggers, et al.,
bioRxiv - Biophysics 2021
Quote:
... and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
-
No products found
because this supplier's products are not listed.
Luke F. Nunnelly, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... and St18 (1:1000) were incubated in 5% NDS/PBS overnight at 4°C and secondary antibodies (Jackson Immunoresearch, 1:1000) diluted in 0.01% Triton X-100/PBS for 2 hours at room temperature ...
-
No products found
because this supplier's products are not listed.
Sunil Yeruva, et al.,
bioRxiv - Cell Biology 2023
Quote:
... diluted 1:50 in 5 % BSA/TBST overnight at 4°C (BSA: VWR; #422361V). As a negative control ...
-
No products found
because this supplier's products are not listed.
Victoria Gonzalez, et al.,
bioRxiv - Microbiology 2024
Quote:
... Cells were probed overnight at 4°C for SEOV N protein (custom, Genscript) at dilution 1:400 in 1xPBS and with secondary antibody AlexaFluor 555 goat α mouse (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Sabah Mozafari, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Shi/Shi:Rag2-/- mice grafted with control and RRMS hiOLs (n=4 per group) were perfused with 1% PBS followed by a mixture of 4% paraformaldehyde/5% glutaraldehyde (Electron Microscopy Science) in 1% PBS ...
-
No products found
because this supplier's products are not listed.
Lisa Donker, et al.,
bioRxiv - Cell Biology 2021
Quote:
... E-cadherin (DECMA-1, GeneTex; GTX11512, 1:1000 IF), αE-catenin (Enzo ...
-
No products found
because this supplier's products are not listed.
Joshua S. Godwin, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Cells were grown in 6-well plates at 37°C in 5% CO2 using growth media consisting of 4:1 of DMEM/M199 (Corning; Corning, NY, USA) supplemented with 15% fetal bovine serum (Avantor® Seradigm ...
-
No products found
because this supplier's products are not listed.
Helen Barbas, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and preblocked for 1 h at 4°C in 5% normal goat serum (NGS; Vector Laboratories), 5% bovine serum albumin (BSA ...
-
No products found
because this supplier's products are not listed.
Arnau Llobet Rosell, et al.,
bioRxiv - Neuroscience 2022
Quote:
... in PTX and an O/N incubation with the following primary antibodies at 4 °C in blocking solution: 1:500 chicken anti-GFP (Rockland), and 1:150 mouse anti-nc82 (DSHB) ...
-
No products found
because this supplier's products are not listed.
J.A. Black, et al.,
bioRxiv - Cell Biology 2021
Quote:
... at 37 °C in 5% C02 and maintained in 5 µg.mL-1 Hygromycin (InvivoGen) and 5 µg.mL-1 Phleomycin (InvivoGen).
-
No products found
because this supplier's products are not listed.
Rasmus K. Jensen, et al.,
bioRxiv - Immunology 2021
Quote:
... 1:10.000 diluted E-tag-HRP antibody (Bethyl), was added and the plate was incubated for 1 h ...
-
No products found
because this supplier's products are not listed.
Alexandros Sountoulidis, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
-
No products found
because this supplier's products are not listed.
Marie-France Dorion, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and C-X3-C motif chemokine ligand 1 (Peprotech) were also added to the culture ...
-
No products found
because this supplier's products are not listed.
Brian Wu, et al.,
bioRxiv - Pathology 2024
Quote:
Primary human lung fibroblasts from either IPF patients (n=4) or donors with no history of lung fibrosis (n=5) were purchased from Lonza at P2 (Supplementary Table 6) ...
-
No products found
because this supplier's products are not listed.
Mengmeng Jin, et al.,
bioRxiv - Neuroscience 2024
Quote:
... in a feeder free fashion and passaged every 4∼5 days at a 1:4∼1:6 ratio using ReLeSR (Stemcell technologies) as described previously 88,95 ...
-
No products found
because this supplier's products are not listed.
Sydney E. Cason, Erika L.F. Holzbaur,
bioRxiv - Cell Biology 2023
Quote:
All the videos (2 min, 4-5 frames s-1) were acquired at 37°C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
-
No products found
because this supplier's products are not listed.
Leticia Peris, et al.,
bioRxiv - Neuroscience 2021
Quote:
... at 4°C overnight and 1 h with appropriate secondary antibodies (LI-COR Biosciences). Image acquisition was performed with an Odyssey infrared imaging system (LI-COR Biosciences ...
-
No products found
because this supplier's products are not listed.
Pedro L. Moura, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... at 4°C and distributed at 5 mL per LS column (Miltenyi Biotec), or 5×106 BM MNC were thawed ...
-
No products found
because this supplier's products are not listed.
Alexandre Pellan Cheng, et al.,
bioRxiv - Genomics 2022
Quote:
... n = 5 Illumina-sequenced datasets and n = 1 WGS of normal DNA (PBMC, Illumina sequencing). Variants were identified in all datasets using lofreq67 (without filtering ...
-
No products found
because this supplier's products are not listed.
Alba Timón-Gómez, et al.,
bioRxiv - Physiology 2021
Quote:
... for 5 min at 22 °C and mounted in Mowiol 4–88 (Polysciences) with DABCO (25 mg ml−1 ...
-
No products found
because this supplier's products are not listed.
Oumeng Zhang, et al.,
bioRxiv - Biophysics 2022
Quote:
... 1.5 NA TIRF objective lens (OL, Olympus UP-LAPO100XOHR) and an achromatic tube lens (TL ...
-
No products found
because this supplier's products are not listed.
Evan P. Kingsley, et al.,
bioRxiv - Genetics 2021
Quote:
... we dissected embryonic tails at E12.5 (n = 4) and E14.5 (n = 4) and extracted RNA using 50 µl Direct-zol (Zymo Research) following the manufacturer’s protocol and used the same library preparation procedures as for the parental samples ...
-
No products found
because this supplier's products are not listed.
Zarna Rajeshkumar Pala, et al.,
bioRxiv - Microbiology 2023
Quote:
... was recorded every 1 min over a period of 4 hours at 37°C using the Cytation 5 microplate reader (BioTek Instruments, USA).