-
No products found
because this supplier's products are not listed.
Dario Hüsler, et al.,
bioRxiv - Microbiology 2022
Quote:
... The dried remainder of eluate 2 was dissolved in 50 µl dry acetonitrile and 50 µl N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide containing 1% tert-butyldimethylsilyl chloride (Sigma) and kept at 70°C for 30 min ...
-
No products found
because this supplier's products are not listed.
Sadiyah Hanif, et al.,
bioRxiv - Neuroscience 2024
Quote:
5’-bromo-2’-deoxyuridine (BrdU) (Roche, Indianapolis, IN) was dissolved in 0.9% NaCl and injected intraperitoneally (i.p. ...
-
No products found
because this supplier's products are not listed.
Franco F. Roldán Gallardo, et al.,
bioRxiv - Cell Biology 2024
Quote:
... with 10µM 5-Bromo-2’-Deoxyuridine (Invitrogen) being added for the last 6 h of treatments ...
-
No products found
because this supplier's products are not listed.
Pei-Li Tseng, et al.,
bioRxiv - Cell Biology 2024
Quote:
5-bromo-2-deoxyuridine (BrdU) (Merck) immunofluorescence staining was performed to assess the influence of ECM stiffness on MCF10A proliferation ...
-
No products found
because this supplier's products are not listed.
Zhifei Wu, et al.,
bioRxiv - Neuroscience 2024
Quote:
... or 5-bromo-2’ deoxyuridine (BrdU,100mg/kg, Abcam 142567) was intraperitoneally injected to label the cells in the S phase ...
-
No products found
because this supplier's products are not listed.
Gabriel A. Yette, Scott Stewart, Kryn Stankunas,
bioRxiv - Developmental Biology 2021
Quote:
... and 175 μg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Promega). Reactions were stopped with three PBS rinses ...
-
No products found
because this supplier's products are not listed.
Bradley R Corr, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 5′-bromo-2′-deoxyuridine (BrdU) (Cat. #550891; BD Biosciences; RRID:AB_2868906) was then added directly to the well culture media (final concentration 10 µM) ...
-
No products found
because this supplier's products are not listed.
Tudor Selescu, et al.,
bioRxiv - Physiology 2024
Quote:
... 4-(3-chloro-pyridin-2-yl)-piperazine-1-carboxylic acid (4-tert-butyl-phenyl)-amide (BCTC, Tocris #3875) 10 mM in DMSO ...
-
No products found
because this supplier's products are not listed.
Andrew J. Gaetano, Ryan S. King,
bioRxiv - Developmental Biology 2023
Quote:
... animals were soaked in 20 mg/mL 5-bromo-2’-deoxyuridine (VWR) with 3% DMSO in a 6 g/L Instant Ocean salt solution for 2 hours followed by a 4-hour chase prior to processing using the rapid protocol ...
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No products found
because this supplier's products are not listed.
Justin E. Silpe, et al.,
bioRxiv - Microbiology 2021
Quote:
... 40 μg mL−1 5-Bromo-4-Chloro-3-Indolyl-beta-D-Galactosidase (X-gal, Takara Bio), and 500 μM isopropyl beta-D-1-thiogalactopyranoside (IPTG ...
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No products found
because this supplier's products are not listed.
Anne D. Villela, et al.,
bioRxiv - Microbiology 2020
Quote:
... Membranes were washed three times with 1X PBS for 5 min and developed with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate in alkaline phosphatase buffer (Bio-Rad).
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No products found
because this supplier's products are not listed.
Amrita Sule, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... human TERT (LOX-TERT-iresTK; Addgene plasmid #12245), generously provided by Didier Trono ...
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No products found
because this supplier's products are not listed.
Rafael M. Gandra, et al.,
bioRxiv - Microbiology 2023
Quote:
... 2-tert-butyl-1,4-benzoquinone (TBBQ; Cayman Chemical, Ann Arbor, MI), menadione (MND ...
-
No products found
because this supplier's products are not listed.
Heidi M. Blank, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The tert-butyldimethylsilyl (TBDMS) derivatized samples were analyzed with a gas chromatograph connected to a mass spectrometer (GC-MS) (Agilent 7890A and 5975C inert XL Mass Selective Detector) ...
-
No products found
because this supplier's products are not listed.
Martina Pauk, et al.,
bioRxiv - Physiology 2024
Quote:
5-Bromo-2-deoxyuridine (BrdU) assay was performed with BrdU (Santa Cruz Biotechnology) and anti-BrdU antibody (Santa Cruz Biotechnology) ...
-
No products found
because this supplier's products are not listed.
William D Jackson, et al.,
bioRxiv - Immunology 2023
Quote:
C57BL/6 mice were treated twice with topical R848 and then were injected once I.P with 2 mg of (5-bromo-2’-deoxyuridine) BrdU (Biolegend). Bone-marrow (BM ...
-
No products found
because this supplier's products are not listed.
Angela Armento, et al.,
bioRxiv - Cell Biology 2024
Quote:
... bFGF 5 ng/ml (day 2-4, AF-100-18B, Peprotech), activinA 100 ng/ml (day 4-14 ...
-
No products found
because this supplier's products are not listed.
Yun Deng, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... with 5-bromo-4-chloro-3-indolyl-phosphate nitro blue tetrazolium staining (Vector Laboratories, Burlingame, CA, USA). 10 ∼ 30 embryos were used for each probe ...
-
No products found
because this supplier's products are not listed.
Ole A.W. Haabeth, et al.,
bioRxiv - Immunology 2021
Quote:
... After washing wells were incubated with a 5-bromo-4-chloro-3⍰-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (R&D systems, mouse IFNγ Kit Cat # EL485). Plates were scanned and analyzed using ImmunoSpot Microanalyzer.
-
No products found
because this supplier's products are not listed.
Christopher R. Reilly, et al.,
bioRxiv - Genetics 2021
Quote:
... TERT variants were generated using the Q5 Site-Directed Mutagenesis Kit (NEB) and primers designed using NEBase changer (Table S1) ...
-
No products found
because this supplier's products are not listed.
Anastasia Selyutina, et al.,
bioRxiv - Microbiology 2020
Quote:
... supplemented with 5 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Corning, Corning, NY, USA), 50 μg/ml penicillin/streptomycin (Corning) ...
-
No products found
because this supplier's products are not listed.
Han Na Suh, et al.,
bioRxiv - Cell Biology 2021
Quote:
Isolated Tert+ and Tert-cells (≤ 5 cells) were subjected to synthesize the complementary DNA (cDNA) using REPLI-g WTA Single Cell Kit (QIAGEN) and analyzed for gene expression by qRT-PCR ...
-
No products found
because this supplier's products are not listed.
Aaron T. Kuan, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Slices were washed with ddH2O 5 x 5 minutes at 4°C on ice and were placed in aqueous 2% uranyl acetate (EMS, #22400) overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Masahito Yamagata, Wenjun Yan, Joshua R. Sanes,
bioRxiv - Neuroscience 2020
Quote:
... In situ hybridization using nitro-blue tetrazolium and 5-bromo-4-chloro-3′-indolyphosphate and double color in situ hybridization using TSA Plus (PerkinElmer) were performed as previously described (Yamagata et al.,1999 ...
-
No products found
because this supplier's products are not listed.
GaYoung Park, et al.,
bioRxiv - Bioengineering 2024
Quote:
... hydrogels of varying Young’s modulus: 5 kPa (4% 20 kDa 4-arm PEG-NB, Creative PEGworks; 2 mM RGD, Genscript), 12 kPa (5% 40 kDa 8-arm PEG-NB ...
-
No products found
because this supplier's products are not listed.
Han Na Suh, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The population of Tert+:Ki67+ was assessed by FACS (Gallios™ 561, Beckman Coulter).
-
No products found
because this supplier's products are not listed.
Adam T Fishburn, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 4°C for 5 minutes (Eppendorf centrifuge 5810 R, Rotor S-4-104). Cell pellets were washed with 5 mL D-PBS and centrifugation was repeated ...
-
No products found
because this supplier's products are not listed.
Larissa D. Kruse, et al.,
bioRxiv - Cell Biology 2024
Quote:
... cells were seeded on the fibronectin-coated Petri dish (TPP) and kept in the incubator (37 °C and 5% CO2) for 4-8 h in the EGM-2 (Lonza) containing 2% FBS ...
-
No products found
because this supplier's products are not listed.
Suruchi Sethi, et al.,
bioRxiv - Biochemistry 2024
Quote:
... 2 mM TCEP) and incubated for 1 hour at 4°C before loading on Superose 6 5/150 column (GE Healthcare) equilibrated with the same buffer on an AKTA-micro system (GE Healthcare) ...
-
No products found
because this supplier's products are not listed.
Walden Li, Ryan M. Wyllie, Paul A. Jensen,
bioRxiv - Microbiology 2020
Quote:
... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
-
No products found
because this supplier's products are not listed.
Magdalena Ambrożek-Latecka, et al.,
bioRxiv - Immunology 2023
Quote:
... HUVEC/TERT cells were transfected with pSELECT- hASC-GFP (InvivoGen) plasmid by electroporation as previously described [20] with slight modifications ...
-
No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
-
No products found
because this supplier's products are not listed.
Paul Renauer, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 4 x 2 mm (Phenomenex). The eluents were A ...
-
No products found
because this supplier's products are not listed.
Mario Mietzsch, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 3.5 μL was applied to a glow-discharged Quantifoil copper grid with 2 nm continuous carbon support over holes (Quantifoil R 2/4 400 mesh), blotted ...
-
No products found
because this supplier's products are not listed.
Jessica Migliavacca, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Addgene)[70] in a ratio of 5:3:2 using polyethylenimine (24765-2, Polysciences). Virus supernatant was harvested 30 h after transfection ...
-
No products found
because this supplier's products are not listed.
David A. Siegel, et al.,
bioRxiv - Genomics 2022
Quote:
... and DRB3, 4, 5 (exon 2) genes with Fluidigm Access Array (Fluidigm, Singapore) and sequenced on an Illumina MiSeq sequencer (Illumina, San Diego, USA). HLA alleles and genotypes are called using the Omixon HLA Explore (version 2.0.0 ...
-
No products found
because this supplier's products are not listed.
Anthony Flamier, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and passaging every 4-5 days using ReLeSR (StemCell technologies). hES-qualified Matrigel (Corning ...
-
No products found
because this supplier's products are not listed.
Dannielle K. Zierath, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mice (4–5 weeks old; Jackson Labs, Bar Harbor, ME) were group-housed throughout the infection and monitoring period (5/cage) ...
-
No products found
because this supplier's products are not listed.
Steven Tau, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Plates were imaged every 3-4 d on a Cytation 5 (BioTek) with 4x magnification and phase contrast ...
-
No products found
because this supplier's products are not listed.
Youichi Iwai, et al.,
bioRxiv - Neuroscience 2020
Quote:
... A glass micropipette (2 μm tip diameter, 1B150F-4, World Precision Instruments) filled with HEPES-ACSF was placed to an electrode holder with a headstage preamplifier ...
-
No products found
because this supplier's products are not listed.
Wei Zeng, et al.,
bioRxiv - Bioengineering 2024
Quote:
... Antibodies against TERT were purchased from Proteintech Group (Wuhan ...
-
Prepared to contain higher collagenase and caseinase activities. A dialyzed, lyophilized powder.
Cat# LS005282,
1 gm, $246.00
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Chunlei Cang, Boxun Lu, Dejian Ren,
bioRxiv - Physiology 2020
Quote:
... 4 mg collagenase type 2 (Worthington) and 1.5 mg trypsin (Worthington) ...
-
No products found
because this supplier's products are not listed.
Salman Tamaddon-Jahromi, et al.,
bioRxiv - Cell Biology 2023
Quote:
SKOV-3 cells transfected without or with 100nM siRNA and simultaneously treated with 10μM 5-Aza-Cdr for 4 days were seeded into 2-well cell culture inserts (Ibidi, UK). After cell attachment (6h) ...
-
No products found
because this supplier's products are not listed.
Anjali Bajpai, et al.,
bioRxiv - Genetics 2021
Quote:
... Images containing 5 Z-stacks were captured for quantification (100x magnification) on Axioplan-2 using Axiovision version-4 camera (Carl Zeiss). The intensity of fluorescence of FITC and Cy3 was quantified from the projected images using the LSM-FCS Version 3.2SP software (Carl Zeiss ...
-
No products found
because this supplier's products are not listed.
Jamie Guenthoer, et al.,
bioRxiv - Immunology 2024
Quote:
... Omicron BA.4/BA.5 (Sino Biological, cat. 40589-V08H32), Omicron XBB (Sino Biological ...
-
No products found
because this supplier's products are not listed.
Nicolò Mangraviti, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and 5 ng/ml human FGF-2 (Miltenyi Biotec). Then ...
-
No products found
because this supplier's products are not listed.
Francesco Roncato, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Images were acquired at a rate of one frame every 4-5 min for 4 h using an IX83 inverted microscope (Olympus) equipped with UPlanFLN 20x/0.50 Ph1 ∞/0.17/FN 26.5 objective (Olympus) ...
-
No products found
because this supplier's products are not listed.
Chris Y. Cheung, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Cell nucleus was defined and exposed to 405 nm laser light for 4-5 times 200ms bursts at ∼5% 405 nm laser power (TCS SP8 MP, Leica). After photoconversion of Dendra2-RUVBL2 ...
-
No products found
because this supplier's products are not listed.
Victoria A. Trinkaus, et al.,
bioRxiv - Cell Biology 2020
Quote:
... blocking solution consisting of 2 % BSA (Roth) and 4 % donkey serum (Jackson ImmunoResearch) in PBS was added for 30 min at RT ...
-
No products found
because this supplier's products are not listed.
Danyang Zhang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Data were acquired and filtered (at 5 kHz 4-pole Bessel filter) with MultiClamp 700B amplifier (Molecular Devices), digitized with Digidata 1440A (Molecular Devices ...