-
No products found
because this supplier's products are not listed.
Hanna Gałgańska, Łukasz Gałgański,
bioRxiv - Plant Biology 2020
Quote:
... concentrated with Amicon Ultra 10K filters (Millipore, Billerica, MA, USA), aliquoted and stored at −80°C.
-
No products found
because this supplier's products are not listed.
Matthias Schmidt, et al.,
bioRxiv - Microbiology 2021
Quote:
... 4 °C) by dialysis (SnakeSkin Dialysis Tubing, 10k MWCO, Thermo Fisher Scientific). Protein was concentrated in 30k MWCO Amicon Ultra-15 centrifugal filters (MilliporeSigma ...
-
No products found
because this supplier's products are not listed.
Adrien Dufour, et al.,
bioRxiv - Developmental Biology 2023
Quote:
Samples were concentrated using centrifugal filter devices (Amicon-4 10K, Merck) at 4,000 g for 25 min ...
-
No products found
because this supplier's products are not listed.
Grayson Sipe, et al.,
bioRxiv - Neuroscience 2020
Quote:
... GLAST (1:10k, ab181036, Abcam) and β-actin (1:20k ...
-
No products found
because this supplier's products are not listed.
Nadine Nagy, et al.,
bioRxiv - Immunology 2023
Quote:
4-MU (Alfa Aesar, Ward Hill, MA) was pressed into the mouse chow by TestDiet® as previously described36 ...
-
No products found
because this supplier's products are not listed.
Alexia Caillier, et al.,
bioRxiv - Cell Biology 2022
Quote:
... α4 (8440; Cell Signaling Technology, Danvers, MA, USA), Hsp70 (C92F3A-5 ...
-
No products found
because this supplier's products are not listed.
Andrea Salazar, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 1 μL MgSO4 (4 mM; Biolabs, Ipswich, MA), 1.4 μL dNTPs (1.4 mM ...
-
No products found
because this supplier's products are not listed.
Nikolaus Huber, et al.,
bioRxiv - Physiology 2020
Quote:
... we added ∼10K dpm of 3H-Cort (Catalogue number: NET399250UC, lot number: B00025; Perkin Elmer, Waltham, MA, USA), antiserum (MP Biomedicals 07-120016 ...
-
No products found
because this supplier's products are not listed.
Nathaniel Burman, et al.,
bioRxiv - Biochemistry 2024
Quote:
... concentrated (10k MWCO PES Spin-X UF concentrator, Corning), and analyzed via SDS-PAGE (15% acrylamide ...
-
No products found
because this supplier's products are not listed.
Maria Magdalena Klimecka, et al.,
bioRxiv - Biochemistry 2021
Quote:
... samples were concentrated in 10K MWCO centricons (PAL Corporation) (5000rpm [3214xg] at 4°C) and applied on a Superdex200 HiLoad 16/600 column (GE Healthcare) using 25 mM Hepes pH 7.6 ...
-
No products found
because this supplier's products are not listed.
Herminio M Guajardo, Rita J Valentino,
bioRxiv - Neuroscience 2020
Quote:
Adult male (n=4) and female (n=4) Sprague Dawley rats (Charles River, Wilmington, MA) were shipped from the vendor at ~70 days of age ...
-
No products found
because this supplier's products are not listed.
Liangyu Zhang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... beads were spun in a 10K MWCO filter (VWR, Radnor, PA) at room temperature for 10 minutes at 10,000 x g and then washed with 50 mM ammonium bicarbonate ...
-
No products found
because this supplier's products are not listed.
Feng Wang, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... sperm suspensions (50 µl) were fixed with paraformaldehyde 4 % (w/v; EMS, Hatfield, MA) in phosphate buffered saline (PBS ...
-
No products found
because this supplier's products are not listed.
Huixin Yang, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Purified ULP1 protease was added to the eluent overnight at 4°C (pFGET19_Ulp1, Addgene, Watertown, MA). The cleaved product was loaded onto a Ni column (Cytvia HisTrap™ High Performance ...
-
No products found
because this supplier's products are not listed.
Chen Eitan, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Electroporated cells were transferred to two GelTrex coated 100 mm dishes (1K and 10K) in mTeSR medium supplemented with 10 uM ROCK inhibitor (PeproTech, 1293823) and placed into CO2 incubator for 2 days ...
-
No products found
because this supplier's products are not listed.
Andrea Galisova, et al.,
bioRxiv - Bioengineering 2022
Quote:
... emission 662 nm) signals were recorded for 10k gated single-cell events by an S3e Cell Sorter (BioRad, USA). Mean FL-4 fluorescence signal values of RBD+ cells ...
-
No products found
because this supplier's products are not listed.
Nefeli Slavi, et al.,
bioRxiv - Developmental Biology 2022
Quote:
4-Hydroxytamoxifen (4-OHT) (Tocris Bioscience) was dissolved in corn oil (Millipore-Sigma ...
-
No products found
because this supplier's products are not listed.
Alexander Yermanos, et al.,
bioRxiv - Immunology 2020
Quote:
... 1 µl RNAsin Plus RNAse inhibitor (10K, Promega AG), 1 µl Superscript III (200 U/ml ...
-
No products found
because this supplier's products are not listed.
Fabien Duveau, et al.,
bioRxiv - Genetics 2021
Quote:
... and of the 12 mutant strains (4 reporter genes x 3 mutations) was quantified using a Sony MA-900 flow cytometer (the BD Accuri C6 instrument used for other fluorescence assays was not available due to Covid-19 shutdown ...
-
No products found
because this supplier's products are not listed.
Carly R. Mickelson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... mechanically ventilated (2-4 mL tidal volume; VentElite Small Animal Ventilator, Harvard Apparatus, Holliston, MA, USA), and bilaterally vagotomized to prevent mechanosensory feedback from ventilation ...
-
No products found
because this supplier's products are not listed.
Merrit Romeike, et al.,
bioRxiv - Developmental Biology 2021
Quote:
10k cells were seeded per chamber of a µ-Slide 8 Well Chambered Coverslip (ibidi). Cells were differentiated for 48 hours ...
-
No products found
because this supplier's products are not listed.
Sandra Schifferdecker, et al.,
bioRxiv - Microbiology 2022
Quote:
HEK293T cells (4×105) were seeded in a glass coverslip-bottom petri dish (MatTek, MA, USA), cultured for 16 h at 37°C and then co-transfected with pNLC4-3*CA14TAG and pNESPylRS-eRF1dn-tRNA by using calcium phosphate precipitation ...
-
No products found
because this supplier's products are not listed.
Lucía Peña-Pérez, et al.,
bioRxiv - Immunology 2022
Quote:
3-10k cells were FACS sorted into buffer RLT with β-mercaptoethanol and total RNA prepared using RNAeasy Micro (Qiagen) with on column DNase I treatment ...
-
No products found
because this supplier's products are not listed.
Verena Haage, et al.,
bioRxiv - Neuroscience 2024
Quote:
... 10K HMC3 microglia per well were seeded in 80µl of a Agilent Seahorse XF96 Cell Culture Microplate (Agilent Technologies; Cat #: 101085-004) and incubated overnight ...
-
No products found
because this supplier's products are not listed.
Alexis M. Oppman, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Sections were stored at 4 °C until imaging with an Olympus fluorescent microscope (VS120; Olympus Life Science, Waltham, MA).
-
No products found
because this supplier's products are not listed.
Kayla Barekat, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... NY) and 0.26 mM internal standard (4,4-dimethyl-4-silapentane-1-sulfonic acid/DSS, Cambridge Isotope Laboratory, Andover, MA). Briefly ...
-
No products found
because this supplier's products are not listed.
Candice Byers, et al.,
bioRxiv - Genomics 2021
Quote:
... or PermWash (SOX1) prior to incubation with primary antibody (anti-EpCAM at 1/10k, abcam ab71916; anti-SOX1 at 1/300, R&D Systems AF3369) for 45 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Clara Ortega-de San Luis, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 4-hydroxytamoxifen (4-OHT, Santa Cruz) was freshly prepared in oil (mix of sunflower seed oil ...
-
No products found
because this supplier's products are not listed.
Bader M. Jarai, Catherine A. Fromen,
bioRxiv - Bioengineering 2021
Quote:
... or 25 ng/ml Interleukin-4 (IL-4) (Biolegend) were used to stimulate M1 or M2 polarization ...
-
No products found
because this supplier's products are not listed.
Robert T. Schooley, et al.,
bioRxiv - Microbiology 2021
Quote:
... 1.6.2 (SCIEX, Framingham, MA). The calibration curve was obtained by fitting the peak area ratios of the analyte/I.S ...
-
No products found
because this supplier's products are not listed.
Laura Virtanen, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 4°C (Beckman Coulter). The pellet containing lentiviral particles was suspended in the residual medium ...
-
No products found
because this supplier's products are not listed.
Margaret B. Dreishpoon, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 1uL electroporation enhancer and 4 uL PBS (Lonza Biosciences, Lexington, MA). Each cell suspension was added to individual wells of an electroporation cuvette (Lonza Biosciences ...
-
No products found
because this supplier's products are not listed.
Qingguang Zhang, Kyle W. Gheres, Patrick J. Drew,
bioRxiv - Neuroscience 2020
Quote:
... Neural data were amplified 1000x and filtered (0.1 – 10k Hz, DAM80, World Precision Instruments) and then sampled at 30k Hz (PCI-6259 ...
-
No products found
because this supplier's products are not listed.
Yikun Xing, et al.,
bioRxiv - Microbiology 2023
Quote:
... harvested by centrifugation (3,500 × g for 20 min at 4°C, Centrifuge 5702 R, Eppendorf North America, Framingham, MA), and suspended in an equivalent amount of 1 × PBS ...
-
No products found
because this supplier's products are not listed.
Margaret M. Lowe, et al.,
bioRxiv - Immunology 2023
Quote:
... Formalin-fixed paraffin-embedded (FFPE) sections of 4 μm thickness were stained with antibodies specific for CD21 (predilute, clone 2G9, Leica Biosystems, Danvers, MA), CD3 (predilute ...
-
No products found
because this supplier's products are not listed.
Lucas B. Hoffmann, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... 5 µg of sperm DNA from 4 CT and 4 Cort F0 mice were fragmented by centrifugation for 60 s at 7200 rpm using g-TUBES (Covaris, Woburn, MA, USA) to obtain 8 kb-long fragments ...
-
No products found
because this supplier's products are not listed.
Aleksandra Ellert-Miklaszewska, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Strand-specific RNA libraries were prepared for sequencing (3 - 4 biological replicates/treatment) using a KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, MA, USA). Poly-A mRNAs were purified from 100 ng of total RNA using poly-T-oligo-magnetic beads (Kapa Biosystems ...
-
No products found
because this supplier's products are not listed.
Johad Khoury, et al.,
bioRxiv - Cell Biology 2024
Quote:
... (StemCell technologies, Cambridge, MA) with supplements as previously described 30 ...
-
No products found
because this supplier's products are not listed.
Alexandra Blanco, et al.,
bioRxiv - Microbiology 2024
Quote:
... or 30 ng/mL interleukin-4 (IL-4; Miltenyi Biotec) (M2 ...
-
No products found
because this supplier's products are not listed.
Sarah Smith, Jessica Larsen,
bioRxiv - Pathology 2020
Quote:
... ReadyProbes™ Secondary Antibody conjugated to Alexa Fluor 594 (ThermoFisher, Cat. Num R37119, Waltham, MA were used for immunofluorescence. Fixation occur with 4% paraformaldehyde (PFA) (Ted Pella, Cat. Num. 18505, Redding, CA) or glutaraldhyde (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Venu Thatikonda, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... tumor fragments (4 × 4 × 4 mm3) were implanted on the right hind flanks of NSG female mice (Jackson Laboratory) and allowed to grow to an average volume of 250–350 mm3 as monitored by caliper measurements ...
-
No products found
because this supplier's products are not listed.
Ewan Phillip Ramsay, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Rpc5-WH3/4 and Rpc5-WH1/4 from its genomic DNA (Genscript). The constructs were subsequently cloned into pOPINF or pOPINJ plasmids for bacterial expression or into pACEBac1 plasmid for baculovirus–insect cells expression ...
-
No products found
because this supplier's products are not listed.
Nghi D. P. Dang, et al.,
bioRxiv - Neuroscience 2024
Quote:
... They were fixed overnight at 4°C in 4% paraformaldehyde (PFA) (Polysciences) diluted in 1X Phosphate Buffered Saline ...
-
No products found
because this supplier's products are not listed.
Astrid M. Alsema, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 4 U RNAse inhibitor (Takara, 2313A), 10mM dNTPs (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
William B. Lynch, et al.,
bioRxiv - Neuroscience 2024
Quote:
... at 4degC and then a secondary antibody (Jackson Immunoresearch; 1:10K) for 1 hr ...
-
No products found
because this supplier's products are not listed.
Wander Van Breedam, et al.,
bioRxiv - Bioengineering 2021
Quote:
... from Bio-Rad (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels ...
-
No products found
because this supplier's products are not listed.
Kunyan Zhou, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... and glucose transporter 4 (GLUT4) from Proteintech Group Inc ...
-
No products found
because this supplier's products are not listed.
Emily M. Greeson, et al.,
bioRxiv - Bioengineering 2022
Quote:
Transmission Electron Microscopy (TEM; JEM-1400Flash, JEOL, MA USA) was used to confirm external associations of SPIONs with B ...
-
No products found
because this supplier's products are not listed.
Ben J. Hardcastle, et al.,
bioRxiv - Neuroscience 2020
Quote:
... They were finally washed 4 × 15 min in RT 0.3% PBT and placed in VectaShield at 4 °C overnight before imaging (Vector Laboratories). trans-Tango and GRASP analyses required separate staining of neuropil and respective fluorophores due to different incubation times.
-
No products found
because this supplier's products are not listed.
Florian Wilfling, et al.,
bioRxiv - Cell Biology 2020
Quote:
... or holey SiO2 R1/4 TEM grids (Quantifoil) to allow for more robust correlation and cells were plunge frozen on a Vitrobot Mark IV (FEI ...