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No products found
because this supplier's products are not listed.
Anne C. Gilmore, et al.,
bioRxiv - Cancer Biology 2020
Quote:
4-Amino-5-Methylamino-2’,7’-Difluorofluorescein Diacetate (DAF) (ThermoFisher) was used to measure and spatially resolve nitric oxide (NO ...
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No products found
because this supplier's products are not listed.
Brian H. Kim, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 2-(5-Benzo [1,3] dioxol-5-yl-2-tert-butyl-3HImidazol-4-yl)-6-methylpyridine hydrochloride hydrate (SB505124) (Sigma-Aldrich; St. Louis, MO) and implanted subdermally ...
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No products found
because this supplier's products are not listed.
Anella Saviano, et al.,
bioRxiv - Immunology 2024
Quote:
... and anti-LY6B.2 [7/4] FITC (clone 7/4; Abcam) for 60 min at 4 °C to define neutrophils and monocyte as LY6B.2(7/4)+LY6Ghigh and LY6B.2(7/4)+LY6Glow respectively 32 ...
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No products found
because this supplier's products are not listed.
Lucie Olejníková-Ladislavová, et al.,
bioRxiv - Animal Behavior and Cognition 2024
Quote:
The 5-HT1A antagonist N-[2-[4-(2-Methoxyphenyl)-1-piperazinyl]ethyl]-N-2-pyridinylcyclohexanecarboxamide maleate (WAY100635; Tocris Bioscience) at a dose of 1.0 mg/kg.
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No products found
because this supplier's products are not listed.
Simone Zaghen, et al.,
bioRxiv - Systems Biology 2023
Quote:
... 4 mL of chloroform:isopropanol 2:1 (v:v)(Merck) were used to elute the neutral lipid fraction (NL) ...
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No products found
because this supplier's products are not listed.
Roza Izgilov, et al.,
bioRxiv - Cell Biology 2023
Quote:
using a fluorescent glucose analog, 2-NBDG (2-Deoxy-2-[(7-nitro-2, 1, 3-benzoxadiazol-4-yl) amino]-D-glucose) (11046, Cayman chemical). Cultured 3T3-L1 cells “starved” in a glucose-free medium at 37°C for 1 hr ...
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No products found
because this supplier's products are not listed.
Irene Julca, et al.,
bioRxiv - Plant Biology 2022
Quote:
... The medium was aspirated and fresh medium containing 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, premixed in 5:1 ratio, Promega) was added to the treated cells ...
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No products found
because this supplier's products are not listed.
Christine M. Wardell, et al.,
bioRxiv - Immunology 2024
Quote:
... gently fixed at 4°C for 2-4 hours in Cytofix (diluted 1:4 in PBS; BD biosciences), then embedded in O.C.T ...
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No products found
because this supplier's products are not listed.
Stefano Suzzi, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 4’,6-diamidino-2-phenylindole (1:10,000; Biolegend) was used.
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No products found
because this supplier's products are not listed.
Anurag Kumar Singh, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Myosin Heavy chain 1/2/4/6 (1:1000, Santa Cruz), GAPDH (1:3000 ...
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No products found
because this supplier's products are not listed.
John B. G. Mackey, et al.,
bioRxiv - Immunology 2021
Quote:
... Ly6B.2 (clone 7/4, Bio-Rad), CD133 (clone 13A4 ...
-
No products found
because this supplier's products are not listed.
Martineau Éric, et al.,
bioRxiv - Neuroscience 2020
Quote:
... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5, Dako-Agilent, cat#IR50461-2) followed by 2h at RT with the chicken IgY anti-Neurofilament medium chain (NF-M ...
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No products found
because this supplier's products are not listed.
Ashwin Narayanan, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... concentrations was assessed using reduction in WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]- 1,3-benzene Disulfonate) to water-soluble formazan (Roche, France). Cells were seeded in 96-well plates at 2 × 104 cells/well and treated with the different NAM concentrations at 37°C ...
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No products found
because this supplier's products are not listed.
Wyatt E. Lanik, et al.,
bioRxiv - Cell Biology 2020
Quote:
... with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Corning) supplemented with 10% heat inactivated fetal bovine serum (Sigma) ...
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No products found
because this supplier's products are not listed.
Hui Li, et al.,
bioRxiv - Neuroscience 2020
Quote:
... The membranes were blocked with 5% non-fat dry milk for 2 h at RT and incubated overnight at 4°C with rabbit anti-phospho-ERK1/2 (1:2000, Cell Signaling), anti-phospho-Ser473-Akt (1:2000 ...
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No products found
because this supplier's products are not listed.
B. Vanderperre, et al.,
bioRxiv - Cell Biology 2024
Quote:
... fixed 1 min with 4% paraformaldehyde and stained with 2% uranyl acetate (EMS, 22400-2) for 1 min ...
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No products found
because this supplier's products are not listed.
Renchao Chen, et al.,
bioRxiv - Neuroscience 2021
Quote:
... samples were washed four times with 2×SSC and stored at 4 ⁰C in 2×SSC supplemented with 1:1000 murine RNase inhibitor (NEB M0314L) prior to imaging.
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No products found
because this supplier's products are not listed.
Hannah Karp, et al.,
bioRxiv - Bioengineering 2024
Quote:
... for 2 hrs at 4°C in polypropylene tubes (Beckman Coulter). Concentrated Cas9-EDVs were resuspended in Opti-MEM (Gibco ...
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No products found
because this supplier's products are not listed.
Ketki Patil, et al.,
bioRxiv - Microbiology 2024
Quote:
... The cells were incubated overnight at 4°C with Rhodamine-conjugated UEA-1 (Vector Laboratories, RL-1062-2) diluted 1:200 in 5% bovine serum albumin (BSA ...
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No products found
because this supplier's products are not listed.
Analía Lima, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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No products found
because this supplier's products are not listed.
Yuanyuan Wang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... using a TissueLyser (2 × 30 Hz, 3 min at 4°C; QIAGEN). After homogenization ...
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No products found
because this supplier's products are not listed.
Lisa J. Oyston, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Cells were then incubated overnight at 4°C with rabbit anti-TDP-43 (1:500; Proteintech #10782-2-AP) or rabbit anti-FUS (1:500 ...
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No products found
because this supplier's products are not listed.
Eszter Somogyi, et al.,
bioRxiv - Immunology 2020
Quote:
... the media were refreshed and supplemented with 5 ng/mL IL-7 or 5 ng/mL IL-7 and 4 ng/ml IL-2 (R&D Systems), respectively ...
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No products found
because this supplier's products are not listed.
Angela Armento, et al.,
bioRxiv - Cell Biology 2024
Quote:
... bFGF 5 ng/ml (day 2-4, AF-100-18B, Peprotech), activinA 100 ng/ml (day 4-14 ...
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No products found
because this supplier's products are not listed.
Michael Chabot, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (VWR, Radnor, PA), NaCl (VWR) ...
-
No products found
because this supplier's products are not listed.
Cathy C. Garcia, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Slides were incubated at 4°C with primary (Day 1) and secondary (Day 2) antibodies (Jackson ImmunoResearch) overnight in PBT and 5% donkey serum in a humidified chamber and washed three times for 10 min after each antibody incubation with PBT ...
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No products found
because this supplier's products are not listed.
Esteban Flores, et al.,
bioRxiv - Microbiology 2023
Quote:
... 6 µg of 4:2:1:1 transfer:Gag-Pol (pMDLg-pRRE, Addgene):Rev (pRSV-Rev ...
-
No products found
because this supplier's products are not listed.
Florence E. McLean, et al.,
bioRxiv - Microbiology 2024
Quote:
... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) 25 mM (Lonza, 17-737F), gentamicin 25 μg/ml (Lonza ...
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No products found
because this supplier's products are not listed.
Harold Marcotte, et al.,
bioRxiv - Microbiology 2023
Quote:
... BA.2 and BA.4/5 lacking the C-terminal 19 codons (SΔ19) was synthesized by GenScript. The SΔ19 gene of BA.2.75 ...
-
No products found
because this supplier's products are not listed.
Isabel Melchinger, et al.,
bioRxiv - Pathology 2022
Quote:
... Membrane was blocked with 5% non-fat milk in TBST for 2 hrs and probed overnight at 4°C with the primary antibody against TIM-1/KIM-1/HAVCR (#AF1817, Novus Biologicals). After washing the membrane with TBST ...
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No products found
because this supplier's products are not listed.
Takuya Osakada, et al.,
bioRxiv - Neuroscience 2022
Quote:
... guinea pig anti-c-Fos (1:2000, Synaptic Systems, 226-005, Lot #2-10, 2-13), rabbit anti-Vasopressin (1:5000 ...
-
No products found
because this supplier's products are not listed.
Icaro Putinhon Caruso, et al.,
bioRxiv - Biophysics 2021
Quote:
... Samples of 20 μΜ N-NTD or N-NTD-SR consisting of varied protein:nucleic acid molar ratios (8:1, 4:1, 2:1, 1:1, 1:2) were prepared in low-binding microtubes (Eppendorf® LoBind) in the presence of 10% PEG-4000 (w/v ...
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Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...
Cat# LS004174,
100 mg, $42.00
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Chunlei Cang, Boxun Lu, Dejian Ren,
bioRxiv - Physiology 2020
Quote:
... 4 mg collagenase type 2 (Worthington) and 1.5 mg trypsin (Worthington) ...
-
No products found
because this supplier's products are not listed.
Esther Nuebel, et al.,
bioRxiv - Cell Biology 2020
Quote:
... in a ratio of 4:2:1 using polyethylenimine (PEI) (Cat#23966-1, Polysciences, Inc.) (PEI:DNA mass ratio 3:1) ...
-
No products found
because this supplier's products are not listed.
Emily J. Talbot, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 4 µM digitonin) with or without 7 µM 2’,3’-cGAMP (Invivogen). Cells were further washed in PBS and incubated in culture medium until collection ...
-
No products found
because this supplier's products are not listed.
Sawsan S Alamri, et al.,
bioRxiv - Immunology 2021
Quote:
... were coated overnight at 4°C with the SARS-CoV-2 S1 subunit (amino acids 1–685) (Sino Biological, China) at 1 μg/ml in PBS (50 ul/well) ...
-
No products found
because this supplier's products are not listed.
Lucile Hoch, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
-
No products found
because this supplier's products are not listed.
Kiara Freitag, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 4 °C and stained with the APC-labelled ACSA-2 antibody (1:10, Miltenyi Biotec, 130-102-315) in 0.5 % BSA in PBS ...
-
No products found
because this supplier's products are not listed.
Sydney E. Cason, Erika L.F. Holzbaur,
bioRxiv - Cell Biology 2023
Quote:
All the videos (2 min, 4-5 frames s-1) were acquired at 37°C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
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No products found
because this supplier's products are not listed.
Devesh C. Pant, et al.,
bioRxiv - Neuroscience 2022
Quote:
... protein lysate was immunoprecipitated for 2 h at 4°C with the following antibodies: mouse anti-GFP (1:1000; Takara Bio), mouse anti-FLAG (1:1000 ...
-
No products found
because this supplier's products are not listed.
Manuel Peter, et al.,
bioRxiv - Neuroscience 2020
Quote:
Male 2-4 month old C57BL/6J mice (Charles River) were stereotaxically implanted with 26GA guide cannulas bilaterally (PlasticsOne ...
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No products found
because this supplier's products are not listed.
Mai-Anh T. Vu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... or Drd1-cre (Figures 1, 2, 7, Jackson labs, strain #030329) ages 11 to 24 weeks were injected with AAVs to express genetically encoded proteins for optical measurements and manipulations (see Supplementary Table 1) ...
-
No products found
because this supplier's products are not listed.
Biren M. Dave, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... differentiating cells were confluent again and split 1:3-1:4 into Step 2 differentiation medium: BrainPhys Neuronal Medium (STEMCELL Technologies, cat# 05790), 1X N2 ...
-
No products found
because this supplier's products are not listed.
Olaf Klingbeil, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... LATS1/2 (GeneTex, GTX87014, 1:1,000), p-LATS1/2 (T1079/T1041 ...
-
No products found
because this supplier's products are not listed.
Pawel Mikulski, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 2 or 4 nM concentration and run on NextSeq 550 sequencer (Illumina) with NextSeq 500/550 High Output v2.5 (75 cycles PE ...
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No products found
because this supplier's products are not listed.
Kirsten J. Meyer, Justin R. Nodwell,
bioRxiv - Microbiology 2023
Quote:
... supernatant or size exclusion purified vesicles were extracted with butanol (1:2) overnight at 4°C and the butanol fraction transferred to microtiter plates for absorbance measurement (Synergy, Biotek).
-
No products found
because this supplier's products are not listed.
Marco Schade, et al.,
bioRxiv - Paleontology 2022
Quote:
... 2 & 3 (Figs. 1–3; Supplementary Figs. 1–4) was performed using a Metrotom 1500 (Carl Zeiss Microscopy GmbH, Jena, Germany) in a subsidiary of Zeiss in Essingen ...
-
No products found
because this supplier's products are not listed.
Zhiyong He, Weiqi Qiu, Huiqing Zhou,
bioRxiv - Biochemistry 2024
Quote:
... at 37 °C for specific time (20 min, 1 hour, 2 hours, 4 hours, or 16 hours) followed by Oligo Clean & Concentrator Kits (OCC, Zymo Research, D4061) cleanup ...
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No products found
because this supplier's products are not listed.
Poonam Roshan, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... NFIB (1:2000 in 2% milk, Bethyl), Snail (1:1000 ...
-
No products found
because this supplier's products are not listed.
Carmen Alicia Rivera Pérez, et al.,
bioRxiv - Microbiology 2021
Quote:
... and on cloudy days from 284 µmol m-2 s-1 to 154 µmol m-2 s-1 (Quantum/Radiometer/Photometer model 185B, LI-COR Inc., Lincoln, NE, USA). The cosms were regularly watered with demineralized water ...