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No products found
because this supplier's products are not listed.
Renad Jabarin, et al.,
bioRxiv - Neuroscience 2020
Quote:
PF-4778574 [N-<(3R,4S)-3-[4-(5-cyano-2-thienyl)phenyl]tetrahydro-2H-pyran-4-yl>propane-2-sulfonamide] was obtained as a powder from Sigma (catalog #PZ0211) and solubilized (2.5 mg/ml ...
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No products found
because this supplier's products are not listed.
Simon Lind, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... as well as the P2Y2R antagonist AR-C118925XX (5-[[5-(2,8-Dimethyl-5H-dibenzo[a,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-N-2H-tetrazol-5-yl-2-furancarboxamide) were obtained from Tocris (Abingdon, UK). Subsequent dilutions of receptor ligand and other reagents were made in Krebs-Ringer Glucose phosphate buffer (KRG ...
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No products found
because this supplier's products are not listed.
Maria-Luisa del Rio, et al.,
bioRxiv - Immunology 2024
Quote:
... and 2 mg/ml 3-[4,5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]2-[4-sulfophenyl]-2H-tetrazolium (PMS, Promega) (1/20 ...
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No products found
because this supplier's products are not listed.
Francis Ledesma, et al.,
bioRxiv - Bioengineering 2023
Quote:
... 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8) was purchased from ThermoFisher Scientific.
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No products found
because this supplier's products are not listed.
Annamarie E. Allen, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells were incubated in treatment media for the indicated period of time and then MTS reagent ((3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) purchased from Abcam, (ab197010 ...
-
No products found
because this supplier's products are not listed.
Tuan Minh Tran, et al.,
bioRxiv - Plant Biology 2020
Quote:
DSF (cis-11-methyl-2-dodecenonic) was purchased from Merck and dissolved in DMSO to obtain 100 mM stock ...
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No products found
because this supplier's products are not listed.
Josette Medicielo, et al.,
bioRxiv - Genomics 2023
Quote:
... and Tetrahydro-2-furoic acid (SC-253674) were obtained from Santa Cruz Biotechnology ...
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No products found
because this supplier's products are not listed.
Ashwin Narayanan, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... concentrations was assessed using reduction in WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]- 1,3-benzene Disulfonate) to water-soluble formazan (Roche, France). Cells were seeded in 96-well plates at 2 × 104 cells/well and treated with the different NAM concentrations at 37°C ...
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No products found
because this supplier's products are not listed.
Luisa Saecker, Hanns Häberlein, Sebastian Franken,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... 1-[2-chloro-6-[[(3-iodophenyl)methyl]amino]-9H-purin-9-yl]-1-deoxy-N-methyl-β-D-ribofuranuronamide (2-Chloro-IB-MECA; Cayman Chemicals, 163042-96-4), [2-Amino-4-[3-(trifluoromethyl)phenyl]-3-thienyl] phenylmethanone (VCP 171 ...
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No products found
because this supplier's products are not listed.
Christine Krammer, et al.,
bioRxiv - Immunology 2021
Quote:
... 8 color (4-2-2) configuration (BD Bioscience, Heidelberg, Germany). The system automatically adjusts spillover values for the compensation of standard fluorochromes ...
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No products found
because this supplier's products are not listed.
Eric Franklin, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... 20% methyl-5-norbornene-2,3-dicarboxylic anhydride and 2% catalyst dimethylbenzylamine (Electron Microscopy Sciences) over four days ...
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No products found
because this supplier's products are not listed.
John B. G. Mackey, et al.,
bioRxiv - Immunology 2021
Quote:
... Ly6B.2 (clone 7/4, Bio-Rad), CD133 (clone 13A4 ...
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No products found
because this supplier's products are not listed.
Muhammad Irfanur Rashid, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and 4 ng/ml FGF-2 (PeproTech, USA) at 37 °C in a humidified atmosphere containing 3% CO2 ...
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No products found
because this supplier's products are not listed.
Yuanxiao Tang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... after incubating the samples with 2 μl of RNase A (Thermo) at 37 °C for 2h and then with 8 μl of proteinase K (NEB) at 55 °C for 4h ...
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No products found
because this supplier's products are not listed.
Yu-Te Yeh, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and the post-nuclear supernatant was loaded onto an 8-step discontinuous sucrose density gradient (HEPES-buffered 0.2–2 M sucrose) and centrifuged at 55,000 rpm for 2h at 4 °C using an MLS50 rotor (Beckman Coulter). Extracellular acidification rate (ECAR ...
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No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
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No products found
because this supplier's products are not listed.
Azharul Islam, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 4′6-diamidino-2-phenylindole hydrochloride (Vector Laboratories). More than 20 randomly selected fields of view per sample were photographed using a WHN10×/22 eyepiece and a 60× objective (field of view is 1.1 mm ...
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No products found
because this supplier's products are not listed.
Dhiraj Kumar Singh, et al.,
bioRxiv - Immunology 2020
Quote:
... Antihuman ACE-2 (R&D Systems, USA, 1:50, 2h at 37°C) was used for identification of ACE-2 ...
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No products found
because this supplier's products are not listed.
Helena Shomar, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
2-methyl tryptophan was quantified by an LC-MS system (Agilent) using an XBridge BEH Amide 2.5 μm (Waters ...
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No products found
because this supplier's products are not listed.
Theodora U. J. Bruun, et al.,
bioRxiv - Biochemistry 2022
Quote:
IQN17 was crystallized at room temperature in a hanging-drop vapor diffusion system by mixing 0.3 μL of 15 mg/mL peptide with 0.3 μL of reservoir solution containing 0.1 M imidazole (pH 8.0) and 35 % 2-methyl-2,4-pentanediol (MPD) (Qiagen). Crystals were seen two days after trays were set up ...
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No products found
because this supplier's products are not listed.
Joshua D. Kerkaert, et al.,
bioRxiv - Microbiology 2021
Quote:
... and 300μL of XTT solution was added to each well (0.5mg/mL XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] (VWR) with 25μM menadione in PBS) ...
-
No products found
because this supplier's products are not listed.
Shunya Kaneko, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 2 mM DTT and 2 μCi/mL S-adenosyl-L-[methyl-14C] methionine (PerkinElmer). After 3h incubation ...
-
No products found
because this supplier's products are not listed.
Mikala C. Mueller, et al.,
bioRxiv - Bioengineering 2024
Quote:
... 4′,6-diamidino-2-phenylindole (DAPI; BioLegend) was diluted 1:10,000 from a 5 mg mL-1 stock solution in deionized water ...
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No products found
because this supplier's products are not listed.
Wyatt E. Lanik, et al.,
bioRxiv - Cell Biology 2020
Quote:
... with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Corning) supplemented with 10% heat inactivated fetal bovine serum (Sigma) ...
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No products found
because this supplier's products are not listed.
Sebastian Tacke, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... plain grids (Quantifoil 2/2) were transferred to the microscope ...
-
No products found
because this supplier's products are not listed.
Florence E. McLean, et al.,
bioRxiv - Microbiology 2024
Quote:
... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) 25 mM (Lonza, 17-737F), gentamicin 25 μg/ml (Lonza ...
-
No products found
because this supplier's products are not listed.
Bethany C. Taylor, et al.,
bioRxiv - Systems Biology 2024
Quote:
... according to the Ovation RRBS Methyl-Seq System 1-16 protocol for the first read and the Read 2 primer (Illumina) for the second read ...
-
No products found
because this supplier's products are not listed.
Elias Adriaenssens, et al.,
bioRxiv - Cell Biology 2024
Quote:
... we expressed the GST-tagged WIPI1/2d/3/4 from a pCAG backbone encoding GST-TEV-WIPI1/2/3/4 (RRID:Addgene_223798; RRID:Addgene_223799; RRID:Addgene_223800; RRID:Addgene_223801). The protein was expressed in FreeStyleTM HEK293F cells ...
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Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...
Cat# LS004176,
1 gm, $215.00
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Chunlei Cang, Boxun Lu, Dejian Ren,
bioRxiv - Physiology 2020
Quote:
... 4 mg collagenase type 2 (Worthington) and 1.5 mg trypsin (Worthington) ...
-
No products found
because this supplier's products are not listed.
Dhiraj Kumar Singh, et al.,
bioRxiv - Immunology 2020
Quote:
... or anti-SARS CoV-2 nucleocapsid (N) antibody (Sino Biologicals, USA, 1:100, 2h at 37°C). Antihuman ACE-2 (R&D Systems ...
-
No products found
because this supplier's products are not listed.
Oju Jeon, et al.,
bioRxiv - Bioengineering 2024
Quote:
... or 4 μg BMP-2 (Genscript, Piscataway, NJ) per mg GMs ...
-
No products found
because this supplier's products are not listed.
Mélody Wintrebert, et al.,
bioRxiv - Genetics 2020
Quote:
... at 60 x magnification (Figs. 2 and 4) or DeltaVision microscope (GE Healthcare) at 100 x magnification (Fig ...
-
No products found
because this supplier's products are not listed.
Soh Ishiguro, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... 2 µL of 2 mM dNTPs (Takara #4025), and 0.6 µL of 100% DMSO (NEB #12611P) ...
-
No products found
because this supplier's products are not listed.
Li Zhenyu, Li Tian, Liu Meisui, Ivanovic Tijana,
bioRxiv - Microbiology 2022
Quote:
... Liposomes consisted of 4:4:2:0.1:2 × 10−4 ratio of 1,2,dioleoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids), 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids) ...
-
No products found
because this supplier's products are not listed.
Pauline Adjibade, Rachid Mazroui,
bioRxiv - Cancer Biology 2024
Quote:
... the lysates were incubated for 2h at 4 °C with GFP-Trap-agarose beads (Proteintech). The beads were then washed in lysis buffer and boiled at 95 °C in Laemmli buffer for 10 min to eluate the bound proteins ...
-
No products found
because this supplier's products are not listed.
Yusaku Koga, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
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No products found
because this supplier's products are not listed.
Zhi-Peng Chen, et al.,
bioRxiv - Biophysics 2021
Quote:
... ~2 mg plasmids premixed with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
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No products found
because this supplier's products are not listed.
Emily J. Talbot, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 4 µM digitonin) with or without 7 µM 2’,3’-cGAMP (Invivogen). Cells were further washed in PBS and incubated in culture medium until collection ...
-
No products found
because this supplier's products are not listed.
Icaro Putinhon Caruso, et al.,
bioRxiv - Biophysics 2021
Quote:
... Samples of 20 μΜ N-NTD or N-NTD-SR consisting of varied protein:nucleic acid molar ratios (8:1, 4:1, 2:1, 1:1, 1:2) were prepared in low-binding microtubes (Eppendorf® LoBind) in the presence of 10% PEG-4000 (w/v ...
-
No products found
because this supplier's products are not listed.
Cristina Esteva-Font, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... DAPI (4’,6-diamidino-2-phenylindole, MP Biomedicals, Solon, OH) was added at 300 nM for 15 min ...
-
No products found
because this supplier's products are not listed.
Evelyn Garlick, et al.,
bioRxiv - Cell Biology 2021
Quote:
Samples were imaged using the Nikon N-SIM-S system (Ti-2 stand, Cairn TwinCam with 2 x Hamamatsu Flash 4 sCMOS cameras, Perfect Focus, Nikon laser bed 488 ...
-
No products found
because this supplier's products are not listed.
Lieke Golbach, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or 2 2-well plates (Ibidi, 80286) were seeded per condition ...
-
No products found
because this supplier's products are not listed.
Shaqed Carasso, et al.,
bioRxiv - Microbiology 2023
Quote:
... 2 µl of 2% rat serum (Stemcell technologies) was added to each well for 15 minutes at room temperature and proceeded to further staining without a washing step.
-
No products found
because this supplier's products are not listed.
Masaharu Somiya, Shun’ichi Kuroda,
bioRxiv - Cell Biology 2021
Quote:
... Synergy 2 (BioTek).
-
No products found
because this supplier's products are not listed.
Sereina O. Sutter, et al.,
bioRxiv - Microbiology 2024
Quote:
... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8 ...
-
No products found
because this supplier's products are not listed.
Youichi Iwai, et al.,
bioRxiv - Neuroscience 2020
Quote:
... A glass micropipette (2 μm tip diameter, 1B150F-4, World Precision Instruments) filled with HEPES-ACSF was placed to an electrode holder with a headstage preamplifier ...
-
No products found
because this supplier's products are not listed.
Suil Kim, Douglas G. McMahon,
bioRxiv - Neuroscience 2022
Quote:
Wild-type C57BL/6J mice (2-4 months old; 000664, Jackson Laboratory) were used for behavioral experiments ...
-
No products found
because this supplier's products are not listed.
Simran Virdi, Sanjay P. Sane,
bioRxiv - Neuroscience 2024
Quote:
... Samples were cleared and mounted in methyl salicylate between 2 coverslips and a metallic spacer for visualization in the confocal microscope (Olympus FV3000; Shinjuku, Japan) The acquired XYZ stacks were processed further in FiJi (ImageJ ...
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No products found
because this supplier's products are not listed.
Victoria A. Trinkaus, et al.,
bioRxiv - Cell Biology 2020
Quote:
... blocking solution consisting of 2 % BSA (Roth) and 4 % donkey serum (Jackson ImmunoResearch) in PBS was added for 30 min at RT ...
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No products found
because this supplier's products are not listed.
Eloise Dries, et al.,
bioRxiv - Developmental Biology 2024
Quote:
Embryos were photographed at day 1 and 4 with a Zeiss Axioplan 2 light microscope (Carl Zeiss, Jena, Germany) equipped with an AxioCam camera and an Olympus BX-51 microscope (Olympus ...