-
No products found
because this supplier's products are not listed.
Joseph Mills, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... 3-(2-Methyl-5-nitro-imidazol-1-yl)-N-(2,2,2-trichloro-1-phenylamino-ethyl)-propionamide) (Sigma-Aldrich SML1503). Drugs were diluted in culture media at treatment.
-
No products found
because this supplier's products are not listed.
Fushun Wang, et al.,
bioRxiv - Neuroscience 2021
Quote:
... N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 5 µM, Tocris); Tetrodotoxin (TTX ...
-
No products found
because this supplier's products are not listed.
Nancy G. Azizian, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... were purchased from Click Chemistry Tools and Tris [(1-benzyl-1H-1, 2, 3-triazol-4-yl)methyl] amine from Fisher Scientific.
-
No products found
because this supplier's products are not listed.
Ludovica Iovino, et al.,
bioRxiv - Neuroscience 2022
Quote:
... were incubated for 10 min at 37 °C in the presence of 10 µM 2-amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile (UCPH-101; Abcam 120309, UK), a specific excitatory amino acid transporter 1 (EAAT1/Glast ...
-
No products found
because this supplier's products are not listed.
Irene Julca, et al.,
bioRxiv - Plant Biology 2022
Quote:
... The medium was aspirated and fresh medium containing 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, premixed in 5:1 ratio, Promega) was added to the treated cells ...
-
No products found
because this supplier's products are not listed.
Alix Thomas, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... 212-2 ([(11R)-2-methyl-11-(morpholin-4-ylmethyl)-9-oxa1-azatricyclo[6.3.1.04,12]dodeca-2,4(12),5,7-tetraen-3-yl]-naphthalen-1-ylmethanone) from Cayman Chemical were dissolved in dimethylsulfoxide (DMSO ...
-
No products found
because this supplier's products are not listed.
Krzysztof Mikołajczyk,
bioRxiv - Biochemistry 2024
Quote:
... and untransfected cells were cultured for 2 weeks in a complete medium containing 3 μM N-[(1R,2R)-1- (2,3-dihydro-1,4-benzodioxin-6-yl)-1-hydroxy-3-pyrrolidin-1-ylpropan-2-yl]nonanamide (Genz-123346) (Merck, Darmstadt, Germany), a glucosylceramide synthase inhibitor [26].
-
No products found
because this supplier's products are not listed.
Elisa Godino, Anne Doerr, Christophe Danelon,
bioRxiv - Synthetic Biology 2021
Quote:
... 1,2-dioleoyl-sn-glycero-3phosphoglycerol (DOPG) and 1′,3′-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol (18:1 CL) were purchased from Avanti Polar Lipids as chloroform solutions.
-
No products found
because this supplier's products are not listed.
Ana Monfort-Vengut, et al.,
bioRxiv - Cancer Biology 2024
Quote:
WNK1 depletion by CRISPR-Cas9 editing was done by using two independent gRNAs targeting Exon-1 (5’-CGCCGACGCTGTGACCGGC-3’) and Exon-4 (5’-ACTTACACTGGTCACGCGA-3’) cloned into pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W backbone vector (Addgene #67974). TET-ON-Cas9 expressing cells were infected and BFP-positive cell FACS sorted ...
-
No products found
because this supplier's products are not listed.
Somtochukwu S. Okafor, et al.,
bioRxiv - Bioengineering 2024
Quote:
... 4-(3-butyl-1-imidazolio)-1-butanesulfonic acid triflate (Santa Cruz Biotechnology) was used as a gelation agent at concentrations 20 – 100 mg/mL ...
-
No products found
because this supplier's products are not listed.
Jasmin van den Heuvel, et al.,
bioRxiv - Cell Biology 2021
Quote:
... si-USP10-1 (5’-UCGCUUUGGAUGGAAGUUCUA-3’, Qiagen), si-USP10-2 (5’-UACGUCAACACCCAUGAUAGA-3’ ...
-
No products found
because this supplier's products are not listed.
Blanca Salazar-Sarasua, et al.,
bioRxiv - Plant Biology 2021
Quote:
... in paraffin-embedded sections (8µm) and color was detected with 5-bromo-4-chloroindol-3-yl phosphate/nitrateblue tetrazolium (BCIP/NBT) (Roche).
-
No products found
because this supplier's products are not listed.
Dana R Kennedy, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Single turnover reactions were carried out as follows: 5 µM Clr4 was preincubated 5 minutes with 1 mM final S-adenosyl-methionine (liquid SAM, 3 2mM, NEB #B9003S), and varying concentrations of pSwi6 or unP Swi6 ...
-
No products found
because this supplier's products are not listed.
Olaf Klingbeil, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... 14-3-3 (Cell Signaling, 8312S, 1:1,000).
-
No products found
because this supplier's products are not listed.
Teresa Neuwirth, et al.,
bioRxiv - Immunology 2024
Quote:
... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
-
No products found
because this supplier's products are not listed.
Alexander Stanton, et al.,
bioRxiv - Genetics 2024
Quote:
... and 1:300 OCT 3/4 primary antibody (BD Biosciences 611202) at 4C for 1 hour ...
-
No products found
because this supplier's products are not listed.
J. Wordsworth, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... collagen 3 (1:500; Proteintech, 22734-1-AP), α-SMA (1:25,000 Sigma #A4416) ...
-
No products found
because this supplier's products are not listed.
Ryuta Niikura, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... 1% P/S and 2 ng/mL human IL-3 (PeproTech, AF-300-23). TF-1 cells52 were cultured in RPMI-1640 medium supplemented with 10% FBS ...
-
No products found
because this supplier's products are not listed.
Gina Partipilo, et al.,
bioRxiv - Bioengineering 2024
Quote:
... disodium;4-[3-pyridin-2-yl-6-(4- sulfonatophenyl)-1,2,4-triazin-5-yl]benzenesulfonate hydrate (Ferrozine, VWR) All media components were autoclaved or sterilized using 0.2 μm PES filters.
-
No products found
because this supplier's products are not listed.
Shimiao Liao, et al.,
bioRxiv - Genomics 2020
Quote:
... eri-1(mg366);gfp::nrde-3 animals were mutagenized by ethyl methanesulfonate (EMS), followed by a clonal screen ...
-
No products found
because this supplier's products are not listed.
He-Yun Hsiao, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... we employed the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) or WST-1 (Water-Soluble Tetrazolium 1) assay from Takara, Japan ...
-
No products found
because this supplier's products are not listed.
M. Eugenia Dieterle, et al.,
bioRxiv - Microbiology 2020
Quote:
... coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS ...
-
No products found
because this supplier's products are not listed.
Stephanie S. Kim, et al.,
bioRxiv - Cancer Biology 2021
Quote:
Cells were grown to 70% confluent in 24-well plates prior to treatment with N-[2-[[3-(4-Bromophenyl)-2-propenyl]amino]ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89; R&D Systems) or 3-(1,3-Benzodioxol-5-ylmethylene)-2-oxo-1-pyrrolidinecarboxaldehyde (KNK437 ...
-
No products found
because this supplier's products are not listed.
Cagney Coomer, et al.,
bioRxiv - Neuroscience 2023
Quote:
Larvae were anesthetized with 0.02% ethyl 3-aminobenzoate methanesulfonate salt (Tricane, (E10521, Ethyl 3-aminobenzoate methanesulfonate) and mounted in 1% low melting point agarose (50100, Lonza) in a 60 mm x 15 mm petri dish ...
-
No products found
because this supplier's products are not listed.
Jiachao Xu, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Cells expressing relatively low levels of the sensor or other proteins were imaged at the bottom surfaces by TIRF microscopy every 1 or 1.5 s for a duration of 3 or 5 min in phenol-free DMEM/F12 (Corning) containing 5% FBS and 20 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Panke Qu, et al.,
bioRxiv - Microbiology 2022
Quote:
... HEK293T cells were transfected with the pNL4-3-inGluc and S constructs in a 2:1 ratio using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) in order to generate viral particles ...
-
No products found
because this supplier's products are not listed.
Juan Qin, et al.,
bioRxiv - Biochemistry 2021
Quote:
... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
-
No products found
because this supplier's products are not listed.
Coralie Hérent, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Cy-3 or Cy-5 (1:500, Jackson ImmunoResearch). Sections were counterstained with a fluorescent Nissl stain (NeuroTrace 435/445 blue ...
-
No products found
because this supplier's products are not listed.
Clément Blot, et al.,
bioRxiv - Microbiology 2023
Quote:
The ROS production by macrophages was measured by chemiluminescence in the presence of 5-amino-2,3-dihydro-1,4-phthalazinedione (luminol) using a thermostatically (37°C) controlled for 1 hr (Envision, PerkinElmer). For nitrite release ...
-
No products found
because this supplier's products are not listed.
Nelson García-Vázquez, et al.,
bioRxiv - Biochemistry 2024
Quote:
... LC-3 (1:5,000, Novus Biological NB100–2220), p21 (1:1,000 ...
-
No products found
because this supplier's products are not listed.
Eleonora Grisard, et al.,
bioRxiv - Cell Biology 2021
Quote:
... rabbit anti-human 14-3-3 1/1000 (EPR6380, GeneTex), rabbit anti-human Lamp1 1/1000 (clone EPR4204 ...
-
No products found
because this supplier's products are not listed.
Aniela Zablocki, et al.,
bioRxiv - Cell Biology 2024
Quote:
... at 1:500 and 3-3’-diamino-benzidine-tetrahydrochloride (Dako, K3468). Stained sections were imaged using an NanoZoomer 2.0HT (Hamamatsu).
-
No products found
because this supplier's products are not listed.
Khiem C. Lam, Quanyi Chen, Romina S. Goldszmid,
bioRxiv - Immunology 2024
Quote:
Bis-(3’-5’)-cyclic dimeric adenosine monophosphate (c-di-AMP) (#tlrl-nacda, InvivoGen) (see Note 3 ...
-
No products found
because this supplier's products are not listed.
Yan Li, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Unc13-3 (1:1000, Synaptic systems, 126303), Synpo1 (1:2000 ...
-
No products found
because this supplier's products are not listed.
Arit Ghosh, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 1 ng/mL IL-3 (STEMCELL Technologies, 78040) and 1% Penicillin-Streptomycin (Gibco 15140122) ...
-
No products found
because this supplier's products are not listed.
Nevena B. Ognjenovic, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... blocked for 1 hour at room temperature in PBS containing 3% normal horse serum (Vector Labs, USA; S-2000). Fixed cells were then incubated with the primary antibody in PBS containing 1% bovine serum albumen (BSA ...
-
No products found
because this supplier's products are not listed.
Christophe Royer, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... 3 to 4 week old CD-1 females (Charles River UK) were injected intraperitoneally with 5 IU of PMSG (Intervet ...
-
No products found
because this supplier's products are not listed.
Trevor A. Zandi, et al.,
bioRxiv - Biophysics 2024
Quote:
... coli and cloned into the 5’BamHI and 3’XhoI sites of pGEX6P-1 (GenScript).
-
No products found
because this supplier's products are not listed.
Marco S. Kaiser, et al.,
bioRxiv - Physiology 2021
Quote:
... 3’ and 5’ adaptors (Illumina) were ligated and the resulting product was reverse transcribed to generate cDNA by PCR ...
-
No products found
because this supplier's products are not listed.
Concepcion Manzano, et al.,
bioRxiv - Plant Biology 2022
Quote:
... 1 cm root segments were embedded in 3% agarose and sectioned using a vibratome (Leica VT1000 S) to produce 150-200 μM thickness sections ...
-
No products found
because this supplier's products are not listed.
David A. Salamanca-Díaz, et al.,
bioRxiv - Systems Biology 2024
Quote:
The stained 1 in 3 cell solution was assessed by flow cytometry (CytoFlex S Flow Cytometer, Beckman Coulter) and the number of singlet (based on FSC-H vs FSC-A) ...
-
No products found
because this supplier's products are not listed.
Thillai V. Sekar, et al.,
bioRxiv - Bioengineering 2023
Quote:
... 1/3 for CD8+ selection (Miltenyi Biotec) and 2/3 for CD4+ subsets (both positive selection for CD4+CD25+cells and negative selection for CD4+CD25- cells Miltenyi Biotec) ...
-
No products found
because this supplier's products are not listed.
Sean C Piantadosi, et al.,
bioRxiv - Neuroscience 2022
Quote:
... tertiary streptavidin conjugated Cy-3 (1:250 Jackson lab)].
-
No products found
because this supplier's products are not listed.
Han Zhu, et al.,
bioRxiv - Cell Biology 2022
Quote:
Samples were incubated on a rotator for 5 min at 4°C and then centrifuged at 500g for 5 min (Eppendorf, 5920R; 4°C, ramp speed of 3/3). Supernatant was removed and pellet was resuspended in sort buffer [1mM EDTA (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Lina Russ, et al.,
bioRxiv - Microbiology 2023
Quote:
... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
-
No products found
because this supplier's products are not listed.
Steven Tau, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Plates were imaged every 3-4 d on a Cytation 5 (BioTek) with 4x magnification and phase contrast ...
-
No products found
because this supplier's products are not listed.
Tyler A. Smith, et al.,
bioRxiv - Microbiology 2021
Quote:
... and supplemented with 1% BSA (w/v) and recorded every 4 s for 220 s using an Eclipse Ti microscope (Nikon) with an enclosure maintained at 37 °C and 5% CO2.
-
No products found
because this supplier's products are not listed.
Nina R. Montoya, et al.,
bioRxiv - Microbiology 2022
Quote:
... The plates were developed with TMB (3, 3’, 5, 5’ - Tetramethylbenzidine) ELISA Peroxidase Substrate (Rockland Immunochemical, Limerick, PA), and absorbance was measured on a plate reader at 655 nm.
-
No products found
because this supplier's products are not listed.
Sattya N. Talukdar, et al.,
bioRxiv - Microbiology 2022
Quote:
... The membranes were washed 3 times for 5 min with PBS and then incubated with a secondary Ab (1:15,000) (LI-COR Biosciences) for 1 hour at RT ...
-
No products found
because this supplier's products are not listed.
Hannah Crane, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Wells were incubated with 160□l of primary antibody diluted in 3% BSA for 1 hour at room temperature or overnight at 4°C as follows: Senataxin (1:1000 dilution, Bethyl, A301 – 104A or 105A), USP11 (1:500 dilution ...